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TriMethyl-Histone H3-K27 Rabbit pAb

E45R00718NDatasheetCOAMSDS
Human, Mouse, Rat, Other (Wide Range)
IF (Human embryonic stem cells, Mouse E16.5 neocortex, Goat sperm, HEK293T, Rice, Gallus gallus, Homo sapiens, Mus musculus)
ChIP (A549, Mouse embryonic stem cells, HeLa, Human, Rice, Gossypium, Oryza sativa, Homo sapiens, Rattus norvegicus)
IHC (Mouse lung tumor, Mus musculus, Sus scrofa, Homo sapiens)
ChIP-qPCR (Mus musculus, Homo sapiens)
IP (Gossypium, Mus musculus)
ChIP-Seq (Zea mays)
WB (Mus musculus, Homo sapiens, Rattus norvegicus)
Q16695
$510/ 100 µl
Quantity
Total: $510

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Product Information

Cite ID#EN5516053
TypeMethylated Antibodies
HostRabbit
Positive controlHeLa, NIH/3T3, H3 protein
IsotypeIgG
SummaryActin is a key regulator of RNA polymerase (Pol) II-dependent transcription. Positive transcription elongation factor b (P-TEFb), a Cdk9/cyclin T1 heterodimer, has been reported to play a critical role in transcription elongation. However, the relationship between actin and P-TEFb is still not clear. In this study, actin was found to interact with Cdk9, a catalytic subunit of P-TEFb, in elongation complexes. Using immunofluorescence and immunoprecipitation assays, Cdk9 was found to bind to G-actin through the conserved Thr-186 in the T-loop. Overexpression and in vitro kinase assays showed that G-actin promotes P-TEFb-dependent phosphorylation of the Pol II C-terminal domain. An in vitro transcription experiment revealed that the interaction between G-actin and Cdk9 stimulated Pol II transcription elongation. ChIP and immobilized template assays indicated that actin recruited Cdk9 to a transcriptional template in vivo and in vitro. Using cytokine IL-6-inducible p21 gene expression system, we revealed that actin recruited Cdk9 to endogenous gene. Moreover, overexpression of actin and Cdk9 increased histone H3 acetylation and acetylized histone H3 binding to a transcriptional template through the interaction with histone acetyltransferase, p300. Taken together, our results suggested that actin participates in transcription elongation by recruiting Cdk9 for phosphorylation of the Pol II C-terminal domain, and the actin-Cdk9 interaction promotes chromatin remodeling.
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Price510$
ImmunogenA synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
PurificationAffinity purification
Gene ID8290
Storage/StabilityStore at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
ChIP-seq 1:20 - 1:100

All Names

AlternameH3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A
📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Certificate of Analysis (PDF)🧪MSDS
Material safety data sheet (PDF)

Conjugated Antibody

This product is also available conjugated. Format: E45R00718N-Conjugate. Price: $485 / Size: 100μl.

E45R00718N-FITC$485 / 100μlE45R00718N-Biotin$485 / 100μlE45R00718N-HRP$485 / 100μlE45R00718N-AP$485 / 100μlE45R00718N-AF350$485 / 100μlE45R00718N-AF405M$485 / 100μlE45R00718N-AF488$485 / 100μlE45R00718N-AF514$485 / 100μlE45R00718N-AF532$485 / 100μlE45R00718N-AF555$485 / 100μlE45R00718N-AF568$485 / 100μlE45R00718N-AF405S$485 / 100μlE45R00718N-AF405L$485 / 100μlE45R00718N-AF546$485 / 100μlE45R00718N-AF594$485 / 100μlE45R00718N-AF610$485 / 100μlE45R00718N-AF635$485 / 100μlE45R00718N-AF647$485 / 100μlE45R00718N-AF680$485 / 100μlE45R00718N-AF700$485 / 100μlE45R00718N-AF750$485 / 100μlE45R00718N-AF790$485 / 100μlE45R00718N-APC$485 / 100μlE45R00718N-PE$485 / 100μlE45R00718N-Cy3$485 / 100μlE45R00718N-Cy5$485 / 100μlE45R00718N-Cy5.5$485 / 100μlE45R00718N-Cy7$485 / 100μlE45R00718N-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.

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TriMethyl-Histone H3-K27 Rabbit pAb | EnoGene Biotech Co. Ltd