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MFI2 Protein, Mouse, Recombinant (His Tag)
Datasheet   

  • Catalog Number:

    E45M53284M2-50

  • Amount:

    50 ug

  • Cite ID#:

    EN5423374

  • Price:

    1047$

  • Type:

    Recombinant Proteins

  • Protein length:

    A DNA sequence encoding the mouse MFI2 (Q9R0R1) (Met 1-Gln 708), without the propeptide, was fused with a C-terminal polyhistidine tag.

  • Expression Host:

    HEK293 Cells

  • Species:

    Mouse

  • Amino acid sequence:

    Met1-Gln708

  • Tag:

    C-His

  • Molecular Weight:

    The secreted recombinant mouse MFI2 comprises 700 amino acids and has a calculated molecular mass of 77.5 kDa. The apparent molecular mass of rmMFI2 is approximately 80 kDa in SDS-PAGE under reducing conditions.

  • Activity:

    At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA.
    When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate.
    We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

  • Endotoxin:

    < 1.0 EU per μg of the protein as determined by the LAL method.

  • Gene Name:

    melanotransferrin

  • Gene symbol:

    MELTF

  • Swiss-Prot No.:

    Q9R0R1

  • Function:

    CD Molecule proteins

  • Summary:

    MELTF (Melanotransferrin, also known as MFI2) is a Protein Coding gene. The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. MELTF is broadly expressed in the salivary gland, kidney, and other tissues. The long non-coding RNA MFI2 antisense RNA is overexpressed in human cancer tissues and its increased expression is associated with the occurrence and metastasis of cancer. lncRNA MFI2 could promote proliferation and migration of osteosarcoma cells by regulating FOXP4 expression, which suggested critical roles of lncRNA MFI2 and FOXP4 in the occurrence and development of human osteosarcoma. Diseases associated with MELTF include Osteogenic Sarcoma and Melanotic Neuroectodermal Tumor.

  • Form:

    Lyophilized

  • Purity:

    > 95 % as determined by SDS-PAGE.

  • Storage/Stability:

    In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
    Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

  • Storage Buffer:

    Lyophilized from sterile PBS, pH 7.4.
    Please contact us for any concerns or special requirements.
    Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
    Please refer to the specific buffer information in the hard copy of CoA.

  • Synonyms:

    CD228 Protein, Mouse; MTf Protein, Mouse

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MFI2 Protein, Mouse, Recombinant (His Tag) | EnoGene Biotech Co. Ltd