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Mouse anti DDDDK-Tag mAb

E45M22053NDatasheetCOAMSDS
WB (Homo sapiens, Arabidopsis thaliana, Drosophila melanogaster, Mus musculus, Oryza sativa, Cyprinus carpio, Sus scrofa, Escherichia coli, 应用, Ctenopharyngodon idellus, Danio rerio, Common carp, Xenopus laevis)
IP (Homo sapiens, Mus musculus, Nicotiana benthamiana, Yeast, Danio rerio, Sus scrofa)
Co-IP (Oryza sativa, Toxoplasma gondii, Homo sapiens)
ChIP (Mus musculus)
IF (Homo sapiens, Danio rerio, Sus scrofa)
Co-IP (Xenopus laevis)
$305/ 100 µl
Quantity
Total: $305

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Product Information

Cite ID#EN5516817
TypeTag and Loading Control Antibodies
HostMouse
Positive control293T
IsotypeIgG
SummaryFLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Price305$
ImmunogenA synthetic peptide corresponding to DDDDK tag.
PurificationAffinity purification
Storage/StabilityStore at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
DilutionWB 1:2000 - 1:5000
IHC 1:50 - 1:500
IF 1:50 - 1:500
IP 1:50 - 1:100

All Names

AlternameDDDDK; DDDDK tag; DDDDK-tag
📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Certificate of Analysis (PDF)🧪MSDS
Material safety data sheet (PDF)

Conjugated Antibody

This product is also available conjugated. Format: E45M22053N-Conjugate. Price: $485 / Size: 100μl.

E45M22053N-FITC$485 / 100μlE45M22053N-Biotin$485 / 100μlE45M22053N-HRP$485 / 100μlE45M22053N-AP$485 / 100μlE45M22053N-AF350$485 / 100μlE45M22053N-AF405M$485 / 100μlE45M22053N-AF488$485 / 100μlE45M22053N-AF514$485 / 100μlE45M22053N-AF532$485 / 100μlE45M22053N-AF555$485 / 100μlE45M22053N-AF568$485 / 100μlE45M22053N-AF405S$485 / 100μlE45M22053N-AF405L$485 / 100μlE45M22053N-AF546$485 / 100μlE45M22053N-AF594$485 / 100μlE45M22053N-AF610$485 / 100μlE45M22053N-AF635$485 / 100μlE45M22053N-AF647$485 / 100μlE45M22053N-AF680$485 / 100μlE45M22053N-AF700$485 / 100μlE45M22053N-AF750$485 / 100μlE45M22053N-AF790$485 / 100μlE45M22053N-APC$485 / 100μlE45M22053N-PE$485 / 100μlE45M22053N-Cy3$485 / 100μlE45M22053N-Cy5$485 / 100μlE45M22053N-Cy5.5$485 / 100μlE45M22053N-Cy7$485 / 100μlE45M22053N-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.

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Mouse anti DDDDK-Tag mAb | EnoGene Biotech Co. Ltd