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ELISA Kits
Chicken HMGB1/High mobility group protein B1 ELISA Kit
Chicken
$1347/ 96T
QuantityTotal: $1347
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Cite ID#EN5521277
TypeChicken ELISA Kits
DescriptionChicken HMGB1/High mobility group protein B1 ELISA Kit is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the quantitative measurement of HMGB1 in biological samples. Our rigorously validated, ready-to-go Chicken HMGB1/High mobility group protein B1 ELISA Kit use a simple protocol that easily integrates into your current processes, allowing you to rapidly and accurately detect HMGB1 in your samples. Appropriate sample types may include Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine.
Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested several times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in several separate assays to assess inter-assay precision. Assays were performed by at least two technicians using two lots of components.
Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested several times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in several separate assays to assess inter-assay precision. Assays were performed by at least two technicians using two lots of components.
Assay typeSandwich
Detection principleSpectrophotometrical
Sample typeThis immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Sensitivity0.062 ng/mL
Assay range0.156-10 ng/mL
Kit componentsAssay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
Other reagents requiredDeionized or distilled water
FunctionMultifunctional redox sensitive protein with various roles in different cellular compartments. Nuclear functions are attributed to fully reduced HGMB1. Associates with chromatin and binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA, DNA-containing cruciforms or bent structures, supercoiled DNA and ZDNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters. Can restructure the canonical nucleosome. Proposed to be an universal biosensor for nucleic acids. May promote inflammatory response to sterile and infectious signals and may be involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm may function as sensor and/or chaperone for immunogenic nucleic acids, and mediate autophagy. May act as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury.
FormELISA Kits
SynonymsHigh mobility group protein 1,HMG-1,HMG1,RCJMB04_15a21
Price1347$
Gene NameHMGB1
Storage/StabilityStore at +4°C. Please refer to protocols.
Conjugated Antibody
This product is also available conjugated. Format: E45K00827-Conjugate. Price: $485 / Size: 100μl.
E45K00827-FITC$485 / 100μlE45K00827-Biotin$485 / 100μlE45K00827-HRP$485 / 100μlE45K00827-AP$485 / 100μlE45K00827-AF350$485 / 100μlE45K00827-AF405M$485 / 100μlE45K00827-AF488$485 / 100μlE45K00827-AF514$485 / 100μlE45K00827-AF532$485 / 100μlE45K00827-AF555$485 / 100μlE45K00827-AF568$485 / 100μlE45K00827-AF405S$485 / 100μlE45K00827-AF405L$485 / 100μlE45K00827-AF546$485 / 100μlE45K00827-AF594$485 / 100μlE45K00827-AF610$485 / 100μlE45K00827-AF635$485 / 100μlE45K00827-AF647$485 / 100μlE45K00827-AF680$485 / 100μlE45K00827-AF700$485 / 100μlE45K00827-AF750$485 / 100μlE45K00827-AF790$485 / 100μlE45K00827-APC$485 / 100μlE45K00827-PE$485 / 100μlE45K00827-Cy3$485 / 100μlE45K00827-Cy5$485 / 100μlE45K00827-Cy5.5$485 / 100μlE45K00827-Cy7$485 / 100μlE45K00827-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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