Product Information
Cite ID#EN5521254
TypeHuman ELISA Kits
Research FieldCell Biology
DescriptionHuman NOX1/NADPH oxidase 1 ELISA Kit is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the quantitative measurement of NOX1 in biological samples. Our rigorously validated, ready-to-go Human NOX1/NADPH oxidase 1 ELISA Kit use a simple protocol that easily integrates into your current processes, allowing you to rapidly and accurately detect NOX1 in your samples. Appropriate sample types may include Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine.
Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested several times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in several separate assays to assess inter-assay precision. Assays were performed by at least two technicians using two lots of components.
Assay typeSandwich
Detection principleSpectrophotometrical
Sample typeThis immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Sensitivity0.31 ng/mL
Assay range0.78-50 ng/mL
Kit componentsAssay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
Other reagents requiredDeionized or distilled water
FunctionNOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain.
FormELISA Kits
SynonymsNOX-1,Mitogenic oxidase 1,MOX-1,NADH/NADPH mitogenic oxidase subunit P65-MOX,NOH-1,MOX1,NOH1