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ELISA Kits
Human SRSF1/Serine/arginine-rich splicing factor 1 ELISA Kit
Human
$1144/ 96T
QuantityTotal: $1144
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Product Information
Cite ID#EN5521252
TypeHuman ELISA Kits
Research FieldEpigenetics
DescriptionHuman SRSF1/Serine/arginine-rich splicing factor 1 ELISA Kit is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the quantitative measurement of SRSF1 in biological samples. Our rigorously validated, ready-to-go Human SRSF1/Serine/arginine-rich splicing factor 1 ELISA Kit use a simple protocol that easily integrates into your current processes, allowing you to rapidly and accurately detect SRSF1 in your samples. Appropriate sample types may include Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine.
Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested several times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in several separate assays to assess inter-assay precision. Assays were performed by at least two technicians using two lots of components.
Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested several times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in several separate assays to assess inter-assay precision. Assays were performed by at least two technicians using two lots of components.
Assay typeSandwich
Detection principleSpectrophotometrical
Sample typeThis immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Sensitivity0.12 ng/mL
Assay range0.312-20 ng/mL
Kit componentsAssay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
Other reagents requiredDeionized or distilled water
FunctionPlays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.
FormELISA Kits
SynonymsAlternative-splicing factor 1,ASF-1,Splicing factor, arginine/serine-rich 1,pre-mRNA-splicing factor SF2, P33 subunit,ASF,SF2,SF2P33,SFRS1,OK/SW-cl.3
Price1144$
Gene NameSRSF1
Storage/StabilityStore at +4°C. Please refer to protocols.
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