Product Information
Cite ID#EN5521190
TypeGeneral ELISA Kits
DescriptionGeneral 25OHVD/25-OH Vitamin D ELISA Kit is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the quantitative measurement of 25OHVD in biological samples. Our rigorously validated, ready-to-go General 25OHVD/25-OH Vitamin D ELISA Kit use a simple protocol that easily integrates into your current processes, allowing you to rapidly and accurately detect 25OHVD in your samples. Appropriate sample types may include Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine.
Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested several times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in several separate assays to assess inter-assay precision. Assays were performed by at least two technicians using two lots of components.
Assay typeCompetitive
Detection principleSpectrophotometrical
Sample typeThis immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Sensitivity1.27nmol/L
Assay range3.12-200nmol/L
Kit componentsAssay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
Other reagents requiredDeionized or distilled water
FunctionLow blood calcidiol (25-hydroxy-vitamin D) can result from avoiding the sun. Deficiency results in impaired bone mineralization, and leads to bone softening diseases.
FormELISA Kits
SynonymsCalcifediol,Calcidiol