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ELISA Kits
Human JAK3/Tyrosine-protein kinase JAK3 ELISA Kit
Human
$1009/ 96T
QuantityTotal: $1009
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Cite ID#EN5521052
TypeHuman ELISA Kits
Research FieldSignal Transduction
DescriptionHuman JAK3/Tyrosine-protein kinase JAK3 ELISA Kit is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the quantitative measurement of JAK3 in biological samples. Our rigorously validated, ready-to-go Human JAK3/Tyrosine-protein kinase JAK3 ELISA Kit use a simple protocol that easily integrates into your current processes, allowing you to rapidly and accurately detect JAK3 in your samples. Appropriate sample types may include Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine.
Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested several times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in several separate assays to assess inter-assay precision. Assays were performed by at least two technicians using two lots of components.
Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested several times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in several separate assays to assess inter-assay precision. Assays were performed by at least two technicians using two lots of components.
Assay typeSandwich
Detection principleSpectrophotometrical
Sample typeThis immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Sensitivity36 pg/mL
Assay range78-5000 pg/mL
Kit componentsAssay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
Other reagents requiredDeionized or distilled water
FunctionNon-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.
FormELISA Kits
SynonymsJanus kinase 3,JAK-3,Leukocyte janus kinase,L-JAK
Price1009$
Gene NameJAK3
Storage/StabilityStore at +4°C. Please refer to protocols.
Conjugated Antibody
This product is also available conjugated. Format: E45K00601-Conjugate. Price: $485 / Size: 100μl.
E45K00601-FITC$485 / 100μlE45K00601-Biotin$485 / 100μlE45K00601-HRP$485 / 100μlE45K00601-AP$485 / 100μlE45K00601-AF350$485 / 100μlE45K00601-AF405M$485 / 100μlE45K00601-AF488$485 / 100μlE45K00601-AF514$485 / 100μlE45K00601-AF532$485 / 100μlE45K00601-AF555$485 / 100μlE45K00601-AF568$485 / 100μlE45K00601-AF405S$485 / 100μlE45K00601-AF405L$485 / 100μlE45K00601-AF546$485 / 100μlE45K00601-AF594$485 / 100μlE45K00601-AF610$485 / 100μlE45K00601-AF635$485 / 100μlE45K00601-AF647$485 / 100μlE45K00601-AF680$485 / 100μlE45K00601-AF700$485 / 100μlE45K00601-AF750$485 / 100μlE45K00601-AF790$485 / 100μlE45K00601-APC$485 / 100μlE45K00601-PE$485 / 100μlE45K00601-Cy3$485 / 100μlE45K00601-Cy5$485 / 100μlE45K00601-Cy5.5$485 / 100μlE45K00601-Cy7$485 / 100μlE45K00601-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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