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ELISA Kits
Human MKI67/Proliferation marker protein Ki-67 ELISA Kit
Human
$975/ 96T
QuantityTotal: $975
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Cite ID#EN5520934
TypeHuman ELISA Kits
DescriptionHuman MKI67/Proliferation marker protein Ki-67 ELISA Kit is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the quantitative measurement of MKI67 in biological samples. Our rigorously validated, ready-to-go Human MKI67/Proliferation marker protein Ki-67 ELISA Kit use a simple protocol that easily integrates into your current processes, allowing you to rapidly and accurately detect MKI67 in your samples. Appropriate sample types may include Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine.
Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested several times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in several separate assays to assess inter-assay precision. Assays were performed by at least two technicians using two lots of components.
Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested several times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in several separate assays to assess inter-assay precision. Assays were performed by at least two technicians using two lots of components.
Assay typeSandwich
Detection principleSpectrophotometrical
Sample typeThis immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Sensitivity0.1 ng/mL
Assay range0.312-20 ng/mL
Kit componentsAssay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
Other reagents requiredDeionized or distilled water
FunctionRequired to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.
FormELISA Kits
SynonymsAntigen identified by monoclonal antibody Ki-67,Antigen KI-67
Price975$
Gene NameMKI67
Storage/StabilityStore at +4°C. Please refer to protocols.
Conjugated Antibody
This product is also available conjugated. Format: E45K00483-Conjugate. Price: $485 / Size: 100μl.
E45K00483-FITC$485 / 100μlE45K00483-Biotin$485 / 100μlE45K00483-HRP$485 / 100μlE45K00483-AP$485 / 100μlE45K00483-AF350$485 / 100μlE45K00483-AF405M$485 / 100μlE45K00483-AF488$485 / 100μlE45K00483-AF514$485 / 100μlE45K00483-AF532$485 / 100μlE45K00483-AF555$485 / 100μlE45K00483-AF568$485 / 100μlE45K00483-AF405S$485 / 100μlE45K00483-AF405L$485 / 100μlE45K00483-AF546$485 / 100μlE45K00483-AF594$485 / 100μlE45K00483-AF610$485 / 100μlE45K00483-AF635$485 / 100μlE45K00483-AF647$485 / 100μlE45K00483-AF680$485 / 100μlE45K00483-AF700$485 / 100μlE45K00483-AF750$485 / 100μlE45K00483-AF790$485 / 100μlE45K00483-APC$485 / 100μlE45K00483-PE$485 / 100μlE45K00483-Cy3$485 / 100μlE45K00483-Cy5$485 / 100μlE45K00483-Cy5.5$485 / 100μlE45K00483-Cy7$485 / 100μlE45K00483-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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