Product Information
Cite ID#EN5520877
TypeRat ELISA Kits
DescriptionRat Cycs/Cytochrome c, somatic ELISA Kit is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the quantitative measurement of Cycs in biological samples. Our rigorously validated, ready-to-go Rat Cycs/Cytochrome c, somatic ELISA Kit use a simple protocol that easily integrates into your current processes, allowing you to rapidly and accurately detect Cycs in your samples. Appropriate sample types may include Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine.
Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested several times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in several separate assays to assess inter-assay precision. Assays were performed by at least two technicians using two lots of components.
Assay typeSandwich
Detection principleSpectrophotometrical
Sample typeThis immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Sensitivity0.167 ng/mL
Assay range0.312-20 ng/mL
Kit componentsAssay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
Other reagents requiredDeionized or distilled water
FunctionPlays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
FormELISA Kits