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ELISA Kits
Human NOS2/Nitric oxide synthase, inducible ELISA Kit
Human
$873/ 96T
QuantityTotal: $873
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Product Information
Cite ID#EN5520667
TypeHuman ELISA Kits
DescriptionHuman NOS2/Nitric oxide synthase, inducible ELISA Kit is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the quantitative measurement of NOS2 in biological samples. Our rigorously validated, ready-to-go Human NOS2/Nitric oxide synthase, inducible ELISA Kit use a simple protocol that easily integrates into your current processes, allowing you to rapidly and accurately detect NOS2 in your samples. Appropriate sample types may include Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine.
Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested several times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in several separate assays to assess inter-assay precision. Assays were performed by at least two technicians using two lots of components.
Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested several times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in several separate assays to assess inter-assay precision. Assays were performed by at least two technicians using two lots of components.
Assay typeSandwich
Detection principleSpectrophotometrical
Sample typeThis immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Sensitivity9.8 pg/mL
Assay range15.6-1000 pg/ml
Kit componentsAssay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
Other reagents requiredDeionized or distilled water
FunctionProduces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed:19688109).
FormELISA Kits
SynonymsHepatocyte NOS,HEP-NOS,Inducible NO synthase,Inducible NOS,NOS type II,Peptidyl-cysteine S-nitrosylase NOS2,NOS2A
Price873$
Gene NameNOS2
Storage/StabilityStore at +4°C. Please refer to protocols.
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