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ELISA Kits
Human PAPPA/Pappalysin-1 ELISA Kit
Human
$873/ 96T
QuantityTotal: $873
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Product Information
Cite ID#EN5520666
TypeHuman ELISA Kits
Research FieldCell Biology
DescriptionHuman PAPPA/Pappalysin-1 ELISA Kit is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the quantitative measurement of PAPPA in biological samples. Our rigorously validated, ready-to-go Human PAPPA/Pappalysin-1 ELISA Kit use a simple protocol that easily integrates into your current processes, allowing you to rapidly and accurately detect PAPPA in your samples. Appropriate sample types may include Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine.
Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested several times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in several separate assays to assess inter-assay precision. Assays were performed by at least two technicians using two lots of components.
Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested several times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in several separate assays to assess inter-assay precision. Assays were performed by at least two technicians using two lots of components.
Assay typeSandwich
Detection principleSpectrophotometrical
Sample typeThis immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Sensitivity31.2 pg/mL
Assay range62.5-4000 pg/mL
Kit componentsAssay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
Other reagents requiredDeionized or distilled water
FunctionMetalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.
FormELISA Kits
SynonymsInsulin-like growth factor-dependent IGF-binding protein 4 protease,IGF-dependent IGFBP-4 protease,Pregnancy-associated plasma protein A,PAPP-A
Price873$
Gene NamePAPPA
Storage/StabilityStore at +4°C. Please refer to protocols.
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