Product Information
Cite ID#EN5520558
TypeHuman ELISA Kits
Research FieldEpigenetics
DescriptionHuman RORC/Nuclear receptor ROR-gamma ELISA Kit is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the quantitative measurement of RORC in biological samples. Our rigorously validated, ready-to-go Human RORC/Nuclear receptor ROR-gamma ELISA Kit use a simple protocol that easily integrates into your current processes, allowing you to rapidly and accurately detect RORC in your samples. Appropriate sample types may include Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine.
Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested several times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in several separate assays to assess inter-assay precision. Assays were performed by at least two technicians using two lots of components.
Assay typeCompetitive
Detection principleSpectrophotometrical
Sample typeThis immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Sensitivity0.078 ng/mL
Assay range0.156-10 ng/mL
Kit componentsAssay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
Other reagents requiredDeionized or distilled water
FunctionIsoform 2: Essential for thymopoiesis and the development of several secondary lymphoid tissues, including lymph nodes and Peyer's patches. Required for the generation of LTi (lymphoid tissue inducer) cells. Regulates thymocyte survival through DNA-binding on ROREs of target gene promoter regions and recruitment of coactivaros via the AF-2. Also plays a key role, downstream of IL6 and TGFB and synergistically with RORA, for lineage specification of uncommitted CD4(+) T-helper (T(H)) cells into T(H)17 cells, antagonizing the T(H)1 program. Probably regulates IL17 and IL17F expression on T(H) by binding to the essential enhancer conserved non-coding sequence 2 (CNS2) in the IL17-IL17F locus. May also play a role in the pre-TCR activation cascade leading to the maturation of alpha/beta T-cells and may participate in the regulation of DNA accessibility in the TCR-J(alpha) locus.
FormELISA Kits
SynonymsNuclear receptor RZR-gamma,Nuclear receptor subfamily 1 group F member 3,RAR-related orphan receptor C,Retinoid-related orphan receptor-gamma,NR1F3,RORG,RZRG