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ABO Protein, Human, Recombinant (hFc Tag)
Datasheet   

  • Catalog Number:

    E45H52229M3-100

  • Amount:

    100 ug

  • Cite ID#:

    EN5422319

  • Price:

    1399$

  • Type:

    Recombinant Proteins

  • Protein length:

    A DNA sequence encoding the human ABO (ADX99264.1) (Arg63-Pro354) was expressed with the Fc region of human IgG1 at the N-terminus.

  • Expression Host:

    HEK293 Cells

  • Species:

    Human

  • Amino acid sequence:

    Arg63-Pro354

  • Tag:

    N-hFc

  • Molecular Weight:

    The recombinant human ABO consists 552 amino acids and predicts a molecular mass of 62.4 kDa.

  • Activity:

    At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA.
    When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate.
    We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

  • Endotoxin:

    < 1.0 EU per μg protein as determined by the LAL method.

  • Gene Name:

    ABO blood group

  • Gene symbol:

    ABO

  • Protein Refseq:

    ADX99264.1

  • Summary:

    ABO (ABO, Alpha 1-3-N-Acetylgalactosaminyltransferase And Alpha 1-3-Galactosyltransferase) is a Protein Coding gene. Homologous glycosyltransferases α-(1→3)-N-acetylgalactosaminyltransferase (GTA) and α-(1→3)-galactosyltransferase (GTB) catalyze the final step in ABO(H) blood group A and B antigen synthesis through sugar transfer from activated donor to the H antigen acceptor. These enzymes have a GT-A fold type with characteristic mobile polypeptide loops that cover the active site upon substrate binding. The homologous glycosyltransferases α-1,3-N-acetylgalactosaminyltransferase (GTA) and α-1,3-galactosyltransferase (GTB) carry out the final synthetic step of the closely related human ABO(H) blood group A and B antigens.

  • Form:

    Lyophilized

  • Purity:

    > 90 % as determined by SDS-PAGE.

  • Storage/Stability:

    In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
    Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

  • Storage Buffer:

    Lyophilized from sterile PBS, pH 7.4.
    Please contact us for any concerns or special requirements.
    Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
    Please refer to the specific buffer information in the hard copy of CoA.

  • Synonyms:

    A3GALNT Protein, Human; A3GALT1 Protein, Human; GTB Protein, Human; NAGAT Protein, Human

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ABO Protein, Human, Recombinant (hFc Tag) | EnoGene Biotech Co. Ltd