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PARP3 Protein, Human, Recombinant (His & GST Tag)
Datasheet   

  • Catalog Number:

    E45H51556I6-100

  • Amount:

    100 ug

  • Cite ID#:

    EN5421646

  • Price:

    1223$

  • Type:

    Recombinant Proteins

  • Protein length:

    A DNA sequence encoding the C-terminal segment of human PARP3 isoform 1 (Q9Y6F1-1) (Met 1-Leu 533) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

  • Expression Host:

    Baculovirus-Insect Cells

  • Species:

    Human

  • Amino acid sequence:

    Met1-Leu533

  • Tag:

    N-GST & His

  • Molecular Weight:

    The recombinant human PARP3/GST chimera consists of 768 amino acids and has a calculated molecular mass of 87.8 kDa. It migrates as an approximately 95 kDa band in SDS-PAGE under reducing conditions.

  • Activity:

    At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA.
    When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate.
    We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

  • Endotoxin:

    < 1.0 EU per μg of the protein as determined by the LAL method.

  • Gene Name:

    poly (ADP-ribose) polymerase family, member 3

  • Gene symbol:

    PARP3

  • Swiss-Prot No.:

    Q9Y6F1-1

  • Summary:

    Poly(ADP-ribose) polymerase 3 (PARP3) is an important member of the PARP family and shares high structural similarities with both PARP1 and PARP2. Poly(ADP-ribose) polymerase 3 (PARP3), a critical player in cellular response to DNA double-strand breaks (DSBs), plays an essential role in the maintenance of genome integrity. The ADP ribosyl transferase [poly(ADP-ribose) polymerase] ARTD3(PARP3) is a newly characterized member of the ARTD(PARP) family that catalyzes the reaction of ADP ribosylation, a key posttranslational modification of proteins involved in different signaling pathways from DNA damage to energy metabolism and organismal memory.

  • Form:

    Lyophilized

  • Purity:

    > 85 % as determined by SDS-PAGE.

  • Storage/Stability:

    In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
    Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

  • Storage Buffer:

    Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly
    Please contact us for any concerns or special requirements.
    Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
    Please refer to the specific buffer information in the hard copy of CoA.

  • Synonyms:

    ADPRT3 Protein, Human; ADPRTL2 Protein, Human; ADPRTL3 Protein, Human; ARTD3 Protein, Human; IRT1 Protein, Human; PADPRT-3 Protein, Human

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  • Price:1223$

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PARP3 Protein, Human, Recombinant (His & GST Tag) | EnoGene Biotech Co. Ltd