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ADAM12 Protein, Human, Recombinant (His Tag)
Datasheet   

  • Catalog Number:

    E45H50476M2-20

  • Amount:

    20 ug

  • Cite ID#:

    EN5420566

  • Price:

    695$

  • Type:

    Recombinant Proteins

  • Protein length:

    A DNA sequence encoding the human ADAM12 isoform 1 (NP_003465.3) extracellular domain (Met 1-Ser 513) was fused with a polyhistidine tag at the C-terminus.

  • Expression Host:

    HEK293 Cells

  • Species:

    Human

  • Amino acid sequence:

    Met1-Ser513

  • Tag:

    C-His

  • Molecular Weight:

    The recombinant human ADAM12 consists of 496 amino acids and predicts a molecular mass of 55.2 kDa. As a result of glycosylation, rhADAM12 migrates as three bands with apparent molecular mass of 27, 55 and 72 kDa corresponding to the propeptide, the mature form and the pro form respectively in SDS-PAGE under reducing conditions.

  • Activity:

    Measured by its binding ability in a functional ELISA.Immobilized human ADAM12-His (Cat: PH50476M2) at 10 μg/ml (100 μl/well) can bind biotinylated mouse FLRG-His (Cat: 10591-M08H) with a linear range of 0.31-1.25 μg/ml.

  • Endotoxin:

    < 1.0 EU per μg of the protein as determined by the LAL method.

  • Gene Name:

    ADAM metallopeptidase domain 12

  • Gene symbol:

    ADAM12

  • Protein Refseq:

    NP_003465.3

  • Function:

    Protease proteins, Enzyme Proteins

  • Summary:

    The ADAMs (a disintegrin and metalloprotease) comprise a family of multidomain proteins with metalloprotease, cell adhesion, and signaling activities. Human ADAM12, which is implicated in diseases such as cancer, is expressed in two splice forms, the transmembrane ADAM12-L and the shorter and soluble ADAM12-S. ADAM12, also known as and Meltrin alpha, is a member of the ADAM protein family, which contains one disintegrin domain, one EGF-like domain and one peptidase M12B domain. ADAM12 is synthesized as a zymogen with the prodomain keeping the metalloprotease inactive through a cysteine-switch mechanism. Maturation and activation of the protease involves the cleavage of the prodomain in the trans-Golgi or possibly at the cell surface by a furin-peptidase. It is a membrane-anchored metalloprotease, which has been implicated in activation-inactivation of growth factors that play an important role in wound healing, including heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF) and IGF binding proteins. ADAM12 may also regulate cell-cell and cell-extracellular matrix contacts through interactions with cell surface receptors - integrins and syndecans - potentially influencing the actin cytoskeleton. Moreover, ADAM12 interacts with several cytoplasmic signaling and adaptor molecules through its intracellular domain, thereby directly transmitting signals to or from the cell interior. These ADAM12-mediated cellular effects appear to be critical events in both biological and pathological processes. In addition to protease activity, ADAM12 possesses cell binding and cell signaling properties. In many studies, ADAM12 overexpression has been correlated with disease, and ADAM12 has been shown to promote tumor growth and progression in cancer. On the other hand, protective effects of ADAM12 in disease have also been reported.

  • Form:

    Lyophilized

  • Purity:

    > 98 % as determined by SDS-PAGE.

  • Storage/Stability:

    In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
    Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

  • Storage Buffer:

    Lyophilized from sterile PBS, pH 7.4.
    Please contact us for any concerns or special requirements.
    Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
    Please refer to the specific buffer information in the hard copy of CoA.

  • Synonyms:

    ADAM12-OT1 Protein, Human; CAR10 Protein, Human; MCMP Protein, Human; MCMPMltna Protein, Human; MLTN Protein, Human; MLTNA Protein, Human

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  • Price:695$

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ADAM12 Protein, Human, Recombinant (His Tag) | EnoGene Biotech Co. Ltd