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Cell Lysates

Apolipoprotein CIII (APOC3) (NM_000040) Human Over-expression Lysate

E45H20260-2DatasheetCOAMSDS
$395/ 20 ug
Quantity
Total: $395

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Product Information

Cite ID#EN5541540
TypeHuman Lysates
Research FieldPPAR signaling pathway
DescriptionTransient overexpression lysate of apolipoprotein C-III (APOC3)
Protein lengthGoldORF Clone [GH36472V]
Expression HostHEK293T
SpeciesHuman
TagC-Myc/DDK
Molecular Weight10.9 kDa
Detection principleDetection Antibody:
Clone LBI4C5, Anti-DDK (FLAG) monoclonal antibody ([AMM08808V])
Kit components1 vial of gene specific transient over-expression cell lysate in RIPA buffer
1 vial of whole HEK293T cell lysate in RIPA buffer
1 vial of 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% glycerol, 0.002% Bromophenol blue, 100mM DTT)
Gene symbolAPOC3
mRNA RefseqNM_000040
Protein RefseqNP_000031
Chromosome location11q23.3
FunctionDruggable Genome, Secreted Protein
FormLiquid
PreparationHEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). Cell lysates were aliquoted and stored at -20°C before shipping.
SynonymsAPOCIII
Price395$
Gene ID345
PathwayPPAR signaling pathway
Storage/StabilityUpon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E45H20260-2-Conjugate. Price: $485 / Size: 100μl.

E45H20260-2-FITC$485 / 100μlE45H20260-2-Biotin$485 / 100μlE45H20260-2-HRP$485 / 100μlE45H20260-2-AP$485 / 100μlE45H20260-2-AF350$485 / 100μlE45H20260-2-AF405M$485 / 100μlE45H20260-2-AF488$485 / 100μlE45H20260-2-AF514$485 / 100μlE45H20260-2-AF532$485 / 100μlE45H20260-2-AF555$485 / 100μlE45H20260-2-AF568$485 / 100μlE45H20260-2-AF405S$485 / 100μlE45H20260-2-AF405L$485 / 100μlE45H20260-2-AF546$485 / 100μlE45H20260-2-AF594$485 / 100μlE45H20260-2-AF610$485 / 100μlE45H20260-2-AF635$485 / 100μlE45H20260-2-AF647$485 / 100μlE45H20260-2-AF680$485 / 100μlE45H20260-2-AF700$485 / 100μlE45H20260-2-AF750$485 / 100μlE45H20260-2-AF790$485 / 100μlE45H20260-2-APC$485 / 100μlE45H20260-2-PE$485 / 100μlE45H20260-2-Cy3$485 / 100μlE45H20260-2-Cy5$485 / 100μlE45H20260-2-Cy5.5$485 / 100μlE45H20260-2-Cy7$485 / 100μlE45H20260-2-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.

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Apolipoprotein CIII (APOC3) (NM_000040) Human Over-expression Lysate | EnoGene Biotech Co. Ltd