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Cell Lysates
GJA1 (NM_000165) Human Over-expression Lysate
$395/ 20 ug
QuantityTotal: $395
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Product Information
Cite ID#EN5541489
TypeHuman Lysates
Research FieldArrhythmogenic right ventricular cardiomyopathy (ARVC), Gap junction
DescriptionTransient overexpression lysate of gap junction protein, alpha 1, 43kDa (GJA1)
Protein lengthGoldORF Clone [GH35230V]
Expression HostHEK293T
SpeciesHuman
TagC-Myc/DDK
Molecular Weight42.8 kDa
Detection principleDetection Antibody:
Clone LBI4C5, Anti-DDK (FLAG) monoclonal antibody ([AMM08808V])
Clone LBI4C5, Anti-DDK (FLAG) monoclonal antibody ([AMM08808V])
Kit components1 vial of gene specific transient over-expression cell lysate in RIPA buffer
1 vial of whole HEK293T cell lysate in RIPA buffer
1 vial of 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% glycerol, 0.002% Bromophenol blue, 100mM DTT)
1 vial of whole HEK293T cell lysate in RIPA buffer
1 vial of 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% glycerol, 0.002% Bromophenol blue, 100mM DTT)
Gene symbolGJA1
mRNA RefseqNM_000165
Protein RefseqNP_000156
Chromosome location6q22.31
FunctionDruggable Genome, Ion Channels: Other, Transmembrane
FormLiquid
PreparationHEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). Cell lysates were aliquoted and stored at -20°C before shipping.
SynonymsAVSD3; CMDR; CX43; EKVP; EKVP3; GJAL; HLHS1; HSS; ODDD; PPKCA
Price395$
Gene ID2697
PathwayArrhythmogenic right ventricular cardiomyopathy (ARVC), Gap junction
Storage/StabilityUpon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
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