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Cell Lysates
Protein phosphatase inhibitor 1 (PPP1R1A) (NM_006741) Human Over-expression Lysate
$395/ 20 ug
QuantityTotal: $395
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Cite ID#EN5539716
TypeHuman Lysates
Research FieldLong-term potentiation
DescriptionTransient overexpression lysate of protein phosphatase 1, regulatory (inhibitor) subunit 1A (PPP1R1A)
Protein lengthGoldORF Clone [GH35190V]
Expression HostHEK293T
SpeciesHuman
TagC-Myc/DDK
Molecular Weight18.9 kDa
Detection principleDetection Antibody:
Clone LBI4C5, Anti-DDK (FLAG) monoclonal antibody ([AMM08808V])
Clone LBI4C5, Anti-DDK (FLAG) monoclonal antibody ([AMM08808V])
Kit components1 vial of gene specific transient over-expression cell lysate in RIPA buffer
1 vial of whole HEK293T cell lysate in RIPA buffer
1 vial of 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% glycerol, 0.002% Bromophenol blue, 100mM DTT)
1 vial of whole HEK293T cell lysate in RIPA buffer
1 vial of 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% glycerol, 0.002% Bromophenol blue, 100mM DTT)
Gene symbolPPP1R1A
mRNA RefseqNM_006741
Protein RefseqNP_006732
Chromosome location12q13.2
FunctionDruggable Genome
FormLiquid
PreparationHEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). Cell lysates were aliquoted and stored at -20°C before shipping.
SynonymsI1; IPP1
Price395$
Gene ID5502
PathwayLong-term potentiation
Storage/StabilityUpon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Conjugated Antibody
This product is also available conjugated. Format: E45H18436-2-Conjugate. Price: $485 / Size: 100μl.
E45H18436-2-FITC$485 / 100μlE45H18436-2-Biotin$485 / 100μlE45H18436-2-HRP$485 / 100μlE45H18436-2-AP$485 / 100μlE45H18436-2-AF350$485 / 100μlE45H18436-2-AF405M$485 / 100μlE45H18436-2-AF488$485 / 100μlE45H18436-2-AF514$485 / 100μlE45H18436-2-AF532$485 / 100μlE45H18436-2-AF555$485 / 100μlE45H18436-2-AF568$485 / 100μlE45H18436-2-AF405S$485 / 100μlE45H18436-2-AF405L$485 / 100μlE45H18436-2-AF546$485 / 100μlE45H18436-2-AF594$485 / 100μlE45H18436-2-AF610$485 / 100μlE45H18436-2-AF635$485 / 100μlE45H18436-2-AF647$485 / 100μlE45H18436-2-AF680$485 / 100μlE45H18436-2-AF700$485 / 100μlE45H18436-2-AF750$485 / 100μlE45H18436-2-AF790$485 / 100μlE45H18436-2-APC$485 / 100μlE45H18436-2-PE$485 / 100μlE45H18436-2-Cy3$485 / 100μlE45H18436-2-Cy5$485 / 100μlE45H18436-2-Cy5.5$485 / 100μlE45H18436-2-Cy7$485 / 100μlE45H18436-2-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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