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Cell Lysates
CDC25B (NM_021873) Human Over-expression Lysate
$395/ 20 ug
QuantityTotal: $395
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Product Information
Cite ID#EN5538934
TypeHuman Lysates
Research FieldCell cycle, MAPK signaling pathway, Progesterone-mediated oocyte maturation
DescriptionTransient overexpression lysate of cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 1
Protein lengthGoldORF Clone [GH37292V]
Expression HostHEK293T
SpeciesHuman
TagC-Myc/DDK
Molecular Weight65 kDa
Detection principleDetection Antibody:
Clone LBI4C5, Anti-DDK (FLAG) monoclonal antibody ([AMM08808V])
Clone LBI4C5, Anti-DDK (FLAG) monoclonal antibody ([AMM08808V])
Kit components1 vial of gene specific transient over-expression cell lysate in RIPA buffer
1 vial of whole HEK293T cell lysate in RIPA buffer
1 vial of 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% glycerol, 0.002% Bromophenol blue, 100mM DTT)
1 vial of whole HEK293T cell lysate in RIPA buffer
1 vial of 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% glycerol, 0.002% Bromophenol blue, 100mM DTT)
Gene symbolCDC25B
mRNA RefseqNM_021873
Protein RefseqNP_068659
Chromosome location20p13
FunctionDruggable Genome, Phosphatase
FormLiquid
PreparationHEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). Cell lysates were aliquoted and stored at -20°C before shipping.
Synonymscell division cycle 25 homolog B (S. pombe); cell division cycle 25B; OTTHUMP00000030137; OTTHUMP00000030138; OTTHUMP00000030139
Price395$
Gene ID994
PathwayCell cycle, MAPK signaling pathway, Progesterone-mediated oocyte maturation
Storage/StabilityUpon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
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