World supplier of antibodies, antigens and kits since 2007
Cell Lysates
G protein alpha inhibitor 1 (GNAI1) (NM_002069) Human Over-expression Lysate
$395/ 20 ug
QuantityTotal: $395
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Cite ID#EN5527284
TypeHuman Lysates
Research FieldAxon guidance, Chemokine signaling pathway, Gap junction, Leukocyte transendothelial migration, Long-term depression, Melanogenesis, Progesterone-mediated oocyte maturation, Tight junction
DescriptionTransient overexpression lysate of guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 (GNAI1)
Protein lengthGoldORF Clone [GH35216V]
Expression HostHEK293T
SpeciesHuman
TagC-Myc/DDK
Molecular Weight40.2 kDa
Detection principleDetection Antibody:
Clone LBI4C5, Anti-DDK (FLAG) monoclonal antibody ([AMM08808V])
Clone LBI4C5, Anti-DDK (FLAG) monoclonal antibody ([AMM08808V])
Kit components1 vial of gene specific transient over-expression cell lysate in RIPA buffer
1 vial of whole HEK293T cell lysate in RIPA buffer
1 vial of 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% glycerol, 0.002% Bromophenol blue, 100mM DTT)
1 vial of whole HEK293T cell lysate in RIPA buffer
1 vial of 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% glycerol, 0.002% Bromophenol blue, 100mM DTT)
Gene symbolGNAI1
mRNA RefseqNM_002069
Protein RefseqNP_002060
Chromosome location7q21.11
FunctionDruggable Genome
FormLiquid
PreparationHEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). Cell lysates were aliquoted and stored at -20°C before shipping.
SynonymsGi
Price395$
Gene ID2770
PathwayAxon guidance, Chemokine signaling pathway, Gap junction, Leukocyte transendothelial migration, Long-term depression, Melanogenesis, Progesterone-mediated oocyte maturation, Tight junction
Storage/StabilityUpon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Conjugated Antibody
This product is also available conjugated. Format: E45H06004-2-Conjugate. Price: $485 / Size: 100μl.
E45H06004-2-FITC$485 / 100μlE45H06004-2-Biotin$485 / 100μlE45H06004-2-HRP$485 / 100μlE45H06004-2-AP$485 / 100μlE45H06004-2-AF350$485 / 100μlE45H06004-2-AF405M$485 / 100μlE45H06004-2-AF488$485 / 100μlE45H06004-2-AF514$485 / 100μlE45H06004-2-AF532$485 / 100μlE45H06004-2-AF555$485 / 100μlE45H06004-2-AF568$485 / 100μlE45H06004-2-AF405S$485 / 100μlE45H06004-2-AF405L$485 / 100μlE45H06004-2-AF546$485 / 100μlE45H06004-2-AF594$485 / 100μlE45H06004-2-AF610$485 / 100μlE45H06004-2-AF635$485 / 100μlE45H06004-2-AF647$485 / 100μlE45H06004-2-AF680$485 / 100μlE45H06004-2-AF700$485 / 100μlE45H06004-2-AF750$485 / 100μlE45H06004-2-AF790$485 / 100μlE45H06004-2-APC$485 / 100μlE45H06004-2-PE$485 / 100μlE45H06004-2-Cy3$485 / 100μlE45H06004-2-Cy5$485 / 100μlE45H06004-2-Cy5.5$485 / 100μlE45H06004-2-Cy7$485 / 100μlE45H06004-2-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
Related Products
Categories by research area
Categories by product type
- Antibodies
- Primary Antibodies
- Tag antibodies
- Internal control antibodies
- Pathological (Immunohistochemistry, IHC) antibodies
- Cell Signal Transduction Research Antibodies
- Secondary Antibody
- Antibody Sampler Kits
- ELISA Kits
- Recombinant Proteins
- Fluorescence Reagents
- Signal Inhibitors and Activators
- Transfection Reagent
- Cell biology kits
- Overexpression Cell Lines
- Cell Lysates
- Purification Resins
- 2019-nCoV & Monkeypox