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Cell Lysates

Upstream Binding Protein 1 (UBP1) (NM_001128160) Human Over-expression Lysate

E45H02267-2DatasheetCOAMSDS
$395/ 20 ug
Quantity
Total: $395

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Product Information

Cite ID#EN5523547
TypeHuman Lysates
DescriptionTransient overexpression lysate of upstream binding protein 1 (LBP-1a) (UBP1), transcript variant 2
Protein lengthGoldORF Clone [GH54921V]
Expression HostHEK293T
SpeciesHuman
TagC-Myc/DDK
Molecular Weight56.3 kDa
Detection principleDetection Antibody:
Clone LBI4C5, Anti-DDK (FLAG) monoclonal antibody ([AMM08808V])
Kit components1 vial of gene specific transient over-expression cell lysate in RIPA buffer
1 vial of whole HEK293T cell lysate in RIPA buffer
1 vial of 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% glycerol, 0.002% Bromophenol blue, 100mM DTT)
Gene symbolUBP1
mRNA RefseqNM_001128160
Protein RefseqNP_001121632
Chromosome location3p22.3
FunctionTranscription Factors
FormLiquid
PreparationHEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). Cell lysates were aliquoted and stored at -20°C before shipping.
SynonymsLBP-1a; LBP-1B; LBP1A; LBP1B
Price395$
Gene ID7342
Storage/StabilityUpon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E45H02267-2-Conjugate. Price: $485 / Size: 100μl.

E45H02267-2-FITC$485 / 100μlE45H02267-2-Biotin$485 / 100μlE45H02267-2-HRP$485 / 100μlE45H02267-2-AP$485 / 100μlE45H02267-2-AF350$485 / 100μlE45H02267-2-AF405M$485 / 100μlE45H02267-2-AF488$485 / 100μlE45H02267-2-AF514$485 / 100μlE45H02267-2-AF532$485 / 100μlE45H02267-2-AF555$485 / 100μlE45H02267-2-AF568$485 / 100μlE45H02267-2-AF405S$485 / 100μlE45H02267-2-AF405L$485 / 100μlE45H02267-2-AF546$485 / 100μlE45H02267-2-AF594$485 / 100μlE45H02267-2-AF610$485 / 100μlE45H02267-2-AF635$485 / 100μlE45H02267-2-AF647$485 / 100μlE45H02267-2-AF680$485 / 100μlE45H02267-2-AF700$485 / 100μlE45H02267-2-AF750$485 / 100μlE45H02267-2-AF790$485 / 100μlE45H02267-2-APC$485 / 100μlE45H02267-2-PE$485 / 100μlE45H02267-2-Cy3$485 / 100μlE45H02267-2-Cy5$485 / 100μlE45H02267-2-Cy5.5$485 / 100μlE45H02267-2-Cy7$485 / 100μlE45H02267-2-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.

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Upstream Binding Protein 1 (UBP1) (NM_001128160) Human Over-expression Lysate | EnoGene Biotech Co. Ltd