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Cell Lysates
Adiponectin (ADIPOQ) (NM_001177800) Human Over-expression Lysate
$395/ 20 ug
QuantityTotal: $395
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Product Information
Cite ID#EN5521865
TypeHuman Lysates
Research FieldAdipocytokine signaling pathway, PPAR signaling pathway, Type II diabetes mellitus
DescriptionTransient overexpression lysate of adiponectin, C1Q and collagen domain containing (ADIPOQ), transcript variant 1
Protein lengthGoldORF Clone [GH58511V]
Expression HostHEK293T
SpeciesHuman
TagC-Myc/DDK
Molecular Weight26.9 kDa
Detection principleDetection Antibody:
Clone LBI4C5, Anti-DDK (FLAG) monoclonal antibody ([AMM08808V])
Clone LBI4C5, Anti-DDK (FLAG) monoclonal antibody ([AMM08808V])
Kit components1 vial of gene specific transient over-expression cell lysate in RIPA buffer
1 vial of whole HEK293T cell lysate in RIPA buffer
1 vial of 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% glycerol, 0.002% Bromophenol blue, 100mM DTT)
1 vial of whole HEK293T cell lysate in RIPA buffer
1 vial of 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% glycerol, 0.002% Bromophenol blue, 100mM DTT)
Gene symbolADIPOQ
mRNA RefseqNM_001177800
Protein RefseqNP_001171271
Chromosome location3q27.3
FunctionDruggable Genome, Secreted Protein
FormLiquid
PreparationHEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). Cell lysates were aliquoted and stored at -20°C before shipping.
SynonymsACDC; ACRP30; ADIPQTL1; ADPN; APM-1; APM1; GBP28
Price395$
Gene ID9370
PathwayAdipocytokine signaling pathway, PPAR signaling pathway, Type II diabetes mellitus
Storage/StabilityUpon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
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