
ELISA plate was coated by 2019-nCoV RBD protein, 100 µL/cell at 2µg/ml. The indirect ELISA analysis was performed by loading 100 µL per well of the anti-2019-nCoV RBD mAb (BS05, # E42020T16f ) at various concentrations, respectively. The plate was incubated for 1 hours at 37 ℃ , then washed 5 times. An anti hFc HRP conjugated mAb at a concentration of 1:2000, 100µL/well was used as the secondary antibody. Again, the plate was incubated for 1 hours at 37 ℃ , then washed 5 times. Detection was