human anti-SARS-CoV-2 S1 mAb(BS04)

ELISA analysis was performed by loading 100 µL per well of the anti-2019-nCoV RBD mAb (BS04，# E42020T16e ) at various concentrations, respectively. The plate was incubated for 1 hours at 37℃, then washed 5 times. An anti hFc HRP conjugated mAb at a concentration of 1:2000, 100µL/well was used as the secondary antibody. Again, the plate was incubated for 1 hours at 37℃, then washed 5 times. Detection was performed using TMB substrate for 10 minutes at room temperature in the