human anti-2019-nCoV S1 mAb（BS03）

ELISA plate was coated by 2019-nCoV RBD protein (CAT# E42020T7), 100 µL/cell at 2µg/ml. The indirect ELISA analysis was performed by loading 100 µL per well of the anti-2019-nCoV S1 mAb (BS03) (CAT# E42020T16d) and human anti-2019-nCoV S1 mAb（S1） (CAT# E42020T16) at various concentrations. The plate was incubated for 1 hours at 37℃, then washed 5 times. An anti hFc HRP conjugated mAb at a concentration of 1:2000, 100µL/well was used as the secondary antibody. Again, the plate was incubated for 1 hours at 37℃, then washed 5 times. Detection was performed using TMB