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2019-nCoV & Monkeypox

human anti-2019-nCoV N mAb(N18-m2)

E42020T15m2DatasheetCOAMSDS
Price on request
Quantity

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Form Or Antibody

Form of Antibody:Liquid

Product Information

Host/Isotope:Humanized
Class:Monoclonal
Type:Antibody
Specificity:Binding to N-ter of 2019-nCoV NP. Do not react with the C-ter fragment of 2019-nCoV NP.
Concentration:1.0 mg/mL(may vary lot by lot. Pls check the tag attached at the tube for more info)
Purity:≥95% as determined by SDS-PAGE
Storage Buffer:PBS
Storage conditions:Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.ELISA plate was coated by 2019-nCoV NP, 100 µL/cell at 2µg/ml. The indirect ELISA analysis was performed by loading 100 µL per well of the N18, N18-m1 and N18-m2 at various concentrations, respectively. The plate was incubated for 1 hours at 37℃, then washed 5 times. An anti hFc HRP conjugated mAb at a concentration of 1:2000, 100µL/well was used as the secondary antibody. Again, the plate was incubated for 1 hours at 37℃, then washed 5 times. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2M sulfuric acid. Signal was read on a spectrophotometer at 450nm.
Purification:Protein A
conjugated mAb at a concentration of 1:2000, 100µL/well was used as the secondary the plate was incubated for 1 hours at 37℃, then washed 5 times. Detection was substrate for 10 minutes at room temperature in the dark. The plate was stopped with Signal was read on a spectrophotometer at 450nm.
conjugated mAb at a concentration of 1:2000, 100µL/well was used as the secondary the plate was incubated for 1 hours at 37℃, then washed 5 times. Detection was substrate for 10 minutes at room temperature in the dark. The plate was stopped with Signal was read on a spectrophotometer at 450nm.
human anti-2019-nCoV N mAb(N18-m2) | EnoGene Biotech Co. Ltd