World supplier of antibodies, antigens and kits since 2007
2019-nCoV & Monkeypox
Price on request
Quantity
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Form Or Antibody
Form of AntibodyLiquid
Product Information
Host/Isotope:Humanized
ClassMonoclonal
TypeAntibody
SpecificityBinding to N-ter of 2019-nCoV NP. Do not react with the C-ter fragment of 2019-nCoV NP.
Concentration1.0 mg/mL（may vary lot by lot. Pls check the tag attached at the tube for more info）Purity:>95% by SDS-PAGE
Storage BufferPBS
Storage conditionsStore at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.ELISA plate was coated by 2019-nCoV NP, 100 µL/cell at 5µg/ml. The indirect ELISA analysis was performed by loading 100 µL per well of the anti-2019-nCoV N mAb (N01) at various concentrations. The plate was incubated for 1 hours at 37℃, then washed 5 times. An anti hFc HRP conjugated mAb at a concentration of 1:2000, 100µL/well was used as the secondary antibody. Again, the plate was incubated for 1 hours at 37℃, then washed 5 times. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2M sulfuric acid. Signal was read on a spectrophotometer at 450 nm.
PurificationProtein A
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