Product Information
DescriptionRecombinant 2019-nCoV 3C-like Proteinase is produced by our E.coli expression system and the target gene encoding Ser1-Gln306 is expressed with a 6His tag at the N-terminus.Known as: M Proteinase; 3CL Proteinase
FormulationSupplied as a 0.2 μm filtered solution of PBS, 1mM EDTA, 10% Glycerol, pH 7.4.
PurityGreater than 95% as determined by reducing SDS-PAGE.Reaction:Dabcyl-KTSAVLQSGFRKME-Edans 3CLP Dabcyl-KTSAVLQ + SGFRKME-EdansDefinition of activity: One unit is 1pmol sgfrkme-edans was catalyzed per minute at pH 7.0 and temperature 37°C.
EndotoxinLess than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Activity≥ 10000 u/mg. Disclaimer: The activity data is for reference only. Not guaranteed. Each researcher should verify the enzyme activity in their own model system.
BackgroundThe viral main proteinase (M pro , also called 3CL pro ), which controls the activities of the coronavirus replication complex. It functions as a cysteine protease engaging in the proteolytic cleavage of the viral precursor polyprotein to a series of functional proteins required for coronavirus replication and is considered as an appealing target for designing anti-SARS agents.