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Point Mutation Rapid Cloning Kit
Price on request
Quantity
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Form Or Antibody
Form of AntibodyName:Point Mutation Rapid Cloning Kit
Storage/Stability-Stored at 20 ° C, valid for 1 year
Avoid dilution and preservation
Avoid dilution and preservation
3. 加入 DpnI * 消化反应液中的模板 DNA
将原基因 100 位氨基酸 L 突变为 I
突变点下游（3`）设计足够碱基，使引物在 PCR 过程中可以与模板顺利退火 添加目的突变碱基（红框内序列） *
将原基因 85 位氨基酸 S 突变为 A，同时 100 位氨基酸 E 突变为 K
1. 突变点下游（3`）设计足够碱基，使引物在 PCR 过程中可以与模板顺利退火 2. 添加目的突变碱基（红框内序列） 3. 突变点上游（5`）设计>10 bp 可退火序列（如图为 10 bp）
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