World supplier of antibodies, antigens and kits since 2007
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One Step Rapid and Seamless PCR Cloning Kit
Price on request
Quantity
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Form Or Antibody
Form of AntibodyName:One Step Rapid and Seamless PCR Cloning Kit
Storage/Stability-Stored at 20 ° C, valid for 1 year
Avoid dilution and preservation
Avoid dilution and preservation
如图使用载体上 BseRI / EcoRV 位点进行片段插入，可酶切回收或用虚线所示引物扩增载体 2. 扩增插入片段： ~1000 bp PCR 上游引物为 Forward 部分+载体对应末端 20 bp（载体左侧虚线框对应互补序列）
5`- GCTCTGGAGGAGGCGGGTCAGGAGGAGGCGGGTCACAC-3` PCR 下游引物为 Reverse 部分+载体对应末端 20 bp（载体右侧虚线框对应互补序列） 5`- CGGCCGCCAGTGTGATGGATCTAGTGATGGTGGTGATG-3` 回收线性化载体、片段，配制以下反应体系，50°C，孵育 45min
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