Cytokeratin 16 Antibody | EnoGene Biotech Co. Ltd

Cytokeratin 16 Antibody
Datasheet

Catalog Number:
E38PA1663
Amount:
100 ul
Source:
Rabbit
Reactivity:
H
Applications:
WB, IHC, IF/IC
Price:
225$
Description:
Rabbit polyclonal antibody to Cytokeratin 16
Immunogen:
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cytokeratin 16. The exact sequence is proprietary.
Purification:
The antibody was purified by immunogen affinity chromatography.
Antibody type:
Polyclonal
Form of Antibody:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:
WB (1/500 - 1/1000), IHC (1/50 - 1/200), IHC (1/50 - 1/200)
Gene_symbol:
KRT16
Altername:
KRT16A; Keratin, type I cytoskeletal 16; Cytokeratin-16; CK-16; Keratin-16; K16
Swiss-Prot No.:
P08779
Storage/Stability:
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Western blot analysis of Cytokeratin 16 expression in HeLa (A) whole cell lysates.
Immunohistochemical analysis of Cytokeratin 16 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Cytokeratin 16 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).