BackgroundCoronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Product Information
Expression systemHKU1, produced in E.coli is a single non-glycosylated polypeptide chain containing 441 amino acids. A fully biologically active molecule has a molecular mass of 49.1 kDa. The apparent molecular mass of the protein is approximately 56 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.
SpeciesHuman
Amino acid sequenceMSYTPGHYAGSRSSSGNRSGILKKTSWADQSERNYQTFNRGRKTQPKFTVSTQPQGNTIPHYSWFSGITQFQKGRDFKFSDGQGVPIAFGVPPSEAKGYWYRHSRRSFKTADGQQKQLLPRWYFYYLGTGPYANASYGESLEGVFWVANHQADTSTPSDVSSRDPTTQEAIPTRFPPGTILPQGYYVEGSGRSASNSRPGSRSQSRGPNNRSLSRSNSNFRHSDSIVKPDMADEIANLVLAKLGKDSKPQQVTKQNAKEIRHKILTKPRQKRTPNKHCNVQQCFGKRGPSQNFGNAEMLKLGTNDPQFPILAELAPTPGAFFFGSKLDLVKRDSEADSPVKDVFELHYSGSIRFDSTLPGFETIMKVLEENLNAYVNSNQNTDSDSLSSKPQRKRGVKQLPEQFDSLNLSAGTQHISNDFTPEDHSLLATLDDPYVEDSVA
Accession #:
YP_173242.1
Molecular Weight49.1kDa. The apparent molecular mass of the protein is approximately 56kDa with 6XHis tag observed by reducing SDS-PAGE.
Purity> 95% as analyzed by SDS-PAGE. The concentration is 0.2 mg/ml.
FormulationPurified protein solution after extensive dialysis against PBS, pH 7.4.
EndotoxinLess than 0.2 EU/μg determined by LAL test.
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.