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RNGTT Rabbit Polyclonal Antibody (C-term)
Datasheet   

  • Catalog Number:

    E28PB4010
  • Amount:

    100ul
  • Price:

    255$
  • Altername:

    mRNA-capping enzyme, HCAP1, HCE, Polynucleotide 5'-triphosphatase, mRNA 5'-triphosphatase, TPase, mRNA guanylyltransferase, GTP--RNA guanylyltransferase, GTase, RNGTT, CAP1A
  • Applications:

    WB, FC
  • Reactivity:

    Human, Rat, Mouse
  • Human Gene Id:

    NP_003791.3
  • Swiss-Prot No.:

    O60942
  • Specificity:

    This RNGTT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 489-516 amino acids from the C-terminal region of human RNGTT.
  • Recommended Dilutions:

    WB~~1:1000;FC~~1:10~50
  • Predicted Molecular Weight:

    69kDa
  • Form of Antibody:

    Liquid
  • Storage/Stability:

    Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Isotype:

    Rabbit IgG
  • Background:

    RNGTT is a bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. The protein catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.
  • Tissue Expression:

    Isoform 1 and isoform 4 (at a lesser extent) are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle
  • Cellular localization:

    Nucleus.
  • All lanes : Anti-RNGTT Antibody (C-term) at 1:1000 dilution
    Lane 1: HL-60 whole cell lysate
    Lane 2: rat testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.

    Observed band size : 69kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

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RNGTT Rabbit Polyclonal Antibody (C-term) | EnoGene Biotech Co. Ltd