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E Cadherin (CDH1) Rabbit Polyclonal Antibody (N-term)
Datasheet   

  • Catalog Number:

    E28PB3958

  • Amount:

    100ul

  • Price:

    255$

  • Altername:

    Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324, E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3, CDH1, CDHE, UVO

  • Applications:

    FC, WB

  • Reactivity:

    Human

  • Human Gene Id:

    NP_004351.1

  • Swiss-Prot No.:

    P12830

  • Specificity:

    This E Cadherin (CDH1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-189 amino acids from the N-terminal region of human E Cadherin (CDH1).

  • Recommended Dilutions:

    WB~~1:2000;FC~~1:10~50

  • Predicted Molecular Weight:

    97kDa

  • Form of Antibody:

    Liquid

  • Storage/Stability:

    Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

  • Isotype:

    Rabbit IgG

  • Background:

    CDH1 is a classical cadherin from the cadherin superfamily. This protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization.

  • Tissue Expression:

    Non-neural epithelial tissues.

  • Cellular localization:

    Cell junction, adherens junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Note=Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane

  • All lanes : Anti-CDH1 Antibody (N-term) at 1:2000 dilution
    Lane 1: 293 whole cell lysate
    Lane 2: A431 whole cell lysate
    Lane 3: A549 whole cell lysate
    Lane 4: DU145 whole cell lysate
    Lane 5: MCF-7 whole cell lysate
    Lane 6: T47D whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

    Predicted band size : 97 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

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