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2019-nCoV & Monkeypox

SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag)

E27D2019-P6DatasheetCOAMSDS
2019nCoVExpression Host:HEK293 Cells
$595/ 50ug
Quantity
Total: $595

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Product Information

NameSARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag)
Purity> 95 % as determined by SDS-PAGE.> 95 % as determined by SEC-HPLCBio Activity：1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag)(ZD2019-P6), the EC50 of SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag) is 15-50 ng/mL. 2. Loaded Human ACE2 Protein (His tag) on SA Biosensor, can bind SARS-CoV-2/2019-nCoV Spike/RBD Protein (RBD, mFc Tag) with an affinity constant of 0.562 nM as determined by Octet RED System.
Endotoxin< 1.0 EU per μg protein as determined by the LAL method.Predicted N terminal: Arg 319Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag) consists of 457 amino acids and predicts a molecular mass of 51.5 kDa.
FormulationLiquid or lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.Stability & Storage: Samples are stable for twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
Gene NameSynonym:coronavirus spike; cov spike; ncov RBD; ncov s1; ncov s2; ncov spike; NCP-CoV RBD; NCP-CoV s1; NCP-CoV s2; NCPCoV Spike; novel coronavirus RBD; novel coronavirus s1; novel coronavirus s2; novel coronavirus spike; RBD; S1; S2; Spike RBDProtein Construction:A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (RBD) (YP_009724390.1) (Arg319-Phe541) was expressed with the Fc region of mouse IgG1 at the C-terminus.
References1. Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.
2. Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.
3. Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.
Price595$
Order: order@enogene.com Support: service@enogene.com Web: www.enogene.com
Order: order@enogene.com Support: service@enogene.com Web: www.enogene.com
Protein Description:The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to
Protein Description:The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to
📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E27D2019-P6-Conjugate. Price: $485 / Size: 100μl.

E27D2019-P6-FITC$485 / 100μlE27D2019-P6-Biotin$485 / 100μlE27D2019-P6-HRP$485 / 100μlE27D2019-P6-AP$485 / 100μlE27D2019-P6-AF350$485 / 100μlE27D2019-P6-AF405M$485 / 100μlE27D2019-P6-AF488$485 / 100μlE27D2019-P6-AF514$485 / 100μlE27D2019-P6-AF532$485 / 100μlE27D2019-P6-AF555$485 / 100μlE27D2019-P6-AF568$485 / 100μlE27D2019-P6-AF405S$485 / 100μlE27D2019-P6-AF405L$485 / 100μlE27D2019-P6-AF546$485 / 100μlE27D2019-P6-AF594$485 / 100μlE27D2019-P6-AF610$485 / 100μlE27D2019-P6-AF635$485 / 100μlE27D2019-P6-AF647$485 / 100μlE27D2019-P6-AF680$485 / 100μlE27D2019-P6-AF700$485 / 100μlE27D2019-P6-AF750$485 / 100μlE27D2019-P6-AF790$485 / 100μlE27D2019-P6-APC$485 / 100μlE27D2019-P6-PE$485 / 100μlE27D2019-P6-Cy3$485 / 100μlE27D2019-P6-Cy5$485 / 100μlE27D2019-P6-Cy5.5$485 / 100μlE27D2019-P6-Cy7$485 / 100μlE27D2019-P6-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.

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SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag) | EnoGene Biotech Co. Ltd