Product Information
NameSARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD, His tag)Synonym: coronavirus spike Protein, 2019-nCoV; cov spike Protein, 2019-nCoV; ncov RBD Protein, 2019-nCoV; ncov s1 Protein, 2019-nCoV; ncov s2 Protein, 2019-nCoV; ncov spike Protein, 2019-nCoV; NCP-CoV RBD Protein, 2019-nCoV; NCP-CoV s1 Protein, 2019-nCoV; NCP-CoV s2 Protein, 2019-nCoV; NCP-CoV Spike Protein, 2019-nCoV; novel coronavirus RBD Protein, 2019-nCoV; novel coronavirus s1 Protein, 2019-nCoV; novel coronavirus s2 Protein, 2019-nCoV; novel coronavirus spike Protein, 2019-nCoV; RBD Protein, 2019-nCoV; S1 Protein, 2019-nCoV; S2 Protein, 2019-nCoV; Spike RBD Protein, 2019-nCoVProtein Construction:Protein Construction: A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (RBD) (YP_009724390.1) (Arg319-Phe541) was expressed with the Fc region of human IgG1 at the C-terminus.
Purity> 95 % as determined by SDS-PAGE.> 95 % as determined by SEC-HPLC.Bio Activity: Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, Fc Tag) (ZD2019-P2), the EC50 of 2019-nCoV Spike Protein (RBD, Fc Tag) is 2-6 ng/mL.Predicted N terminal:Arg 319Molecular Mass:The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, Fc Tag) consists of 461 amino acids and predicts a molecular mass of 51.8 kDa.
FormulationLiquid or lyophilized from sterile PBS, pH 7.4.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.Stability & Storage: Samples are stable for 12 months from date of receipt at -20℃ to -80℃. Recommend to aliquot the protein into smaller quantities for optimal storage.Avoid repeated freeze-thaw cycles