Product Information
NameSARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag)
Purity> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.Bio Activity:Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 10μg/mL (100μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag)(ZD2019-P11), the EC50 of SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag)(ZD2019-P11) is 50-100 ng/mL.Predicted N terminal:Arg 319Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag) consists of 234 amino acids and predicts a molecular mass of 23.54 kDa
FormulationLiquid or lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.Stability & Storage: Samples are stable for 12 months from date of receipt at -20℃ to -80℃. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles
Gene NameSynonym:coronavirus spike Protein, 2019-nCoV; cov spike Protein, 2019-nCoV; ncov RBD Protein, 2019-nCoV; ncov s1 Protein, 2019- nCoV; ncov s2 Protein, 2019-nCoV; ncov spike Protein, 2019-nCoV; NCP-CoV RBD Protein, 2019-nCoV; NCP-CoV s1 Protein, 2019-nCoV; NCP-CoV s2 Protein, 2019-nCoV; NCP-CoV Spike Protein, 2019-nCoV; novel coronavirus RBD Protein, 2019- nCoV; novel coronavirus s1 Protein, 2019-nCoV; novel coronavirus s2 Protein, 2019-nCoV; novel coronavirus spike Protein, 2019-nCoV; RBD Protein, 2019-nCoV; S1 Protein, 2019-nCoV; S2 Protein, 2019-nCoV; Spike RBD Protein, 2019-nCoVProtein Construction:A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (RBD) (YP_009724390.1) (Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus.
ReferencesShen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35. Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.