Product Information
NameSARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD, His tag)
Purity> 90 % as determined by SDS-PAGBio Activity:Immobilized Spike Protein (S1+S2 ECD, His tag) (ZD2019-P1) at156.5-10000ng/mL (100 μL/well) can bind 10ug/ml of Human ACE2, Fc Tag
FormulationLiquid or lyophilized from sterile 20 mM Tris, 300 mM NaCl, 10 % glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.Stability & Storage: Samples are stable for twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles
ReconstitutionDetailed reconstitution instructions are sent along with the products
Gene NameSynonym: coronavirus spike; cov spike; ncov RBD; ncov s1; ncov s2; ncov spike; NCP-CoV RBD; NCP-CoV s1; NCP-CoV s2; NCP-CoV Spike; novelcoronavirus RBD; novel coronavirus s1; novel coronavirus s2; novel coronavirus spike; RBD; S1; S2; Spike RBDProtein Construction:A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD) (YP_009724390.1) (Val16-Pro1213) was expressed with a polyhistidine tag at the C-terminus.
References1.Shen S, et al. (2007) Expression, glycosylation, and modification of thespike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35. 2.Du L,
et al. (2009) The spike protein of SARS-CoV--a target for vaccine and
therapeutic development. Nat Rev Microbiol. 7 (3): 226-36. 3.Xiao X, et al.
(2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.