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Anti-APG1(ULK1) (Center) Rabbit pAb
Datasheet
Catalog Number:
E2616611Amount:
100μg/100μlForm of Antibody:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.Storage/Stability:
Store at -20℃/1 yearImmunogen:
This APG1 (ULK1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 642-672 amino acids from the Central region of human APG1 (ULK1).Reactivity:
HumanApplications:
WB~~1:1000 ICC~~1:100Clone Number:
RB3020Isotype:
. :Rabbit Ig
- Fluorescent image of U251 cells stained with APG1 (ULK1) (Center) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with APG1 (ULK1) (Center) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). APG1 (ULK1) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
- Western blot analysis of anti-ULK1 Pab in A2058 cell lysate. ULK1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Host:
RabbitAffinity:
purified
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