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2019-nCoV & Monkeypox

Recombinant 2019-nCoV Guanine-N7_meth (N-6His)

E21-R83-1DatasheetCOAMSDS
$395/ 10ug
Quantity
Total: $395

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Product Information

DescriptionRecombinant 2019-nCoV Guanine-N7 methyltransferase is produced by our E.coli expression system and the target gene encoding Ala1-Gln527 is expressed with a 6His tag at the N-terminus.Accession #:YP_009725309.1Known as:SARS-CoV 2 nsp14; SARS-CoV 2 ExoN; Guanine-N7 methyltransferase
FormulationSupplied as a 0.2 μm filtered solution of PBS, 10% Glycerol, pH 7.4.Mol Mass:62.9kDaAP Mol Mass:60kDa, reducing conditions.
PurityGreater than 85% as determined by reducing SDS-PAGE.
EndotoxinNA
FormName:Recombinant 2019-nCoV Guanine-N7_meth (C-6His)
Storage/StabilityStore at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.
BackgroundThe nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.positive-stranded RNA genome of the coronaviruses is translated from ORF1 to yield polyproteins that are proteolytically processed into intermediate and mature nonstructural proteins (nsps). SARS-CoV 2 polyproteins incorporate 16 protein domains (nsps).
Price395$
📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

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Recombinant 2019-nCoV Guanine-N7_meth (N-6His) | EnoGene Biotech Co. Ltd