Product Information
DescriptionRecombinant 2019-nCoV Papain-like Protease is produced by our E.coli expression system and the target gene encoding Glu1564-Lys1878 is expressed.Accession #:QHD43415.1Known as:Papain-like Protease; PLpro; PL-PRO; pp1a; Replicase polyprotein 1a
FormulationSupplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 10 mM Mercaptoethanol [BME], 20% Glycerol, pH 7.5.Mol Mass:35.8kDaAP Mol Mass:34kDa, reducing conditions.
PurityGreater than 90% as determined by reducing SDS-PAGE.
BackgroundReplication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro).
Bioactivity:
Measured by its ability to cleave a fluorogenic peptide substrate, (E-EDANS)RELNGGAPI(K-DABCYL)S (Synthetic).
The specific activity is >2000pmoi/min/mg. Disclaimer: The activity data is for reference only. Not guaranteed. Each researcher should verify the enzyme activity in their own model system.