Recombinant 2019-nCoV 3C-like Proteinase (N-6His)
Datasheet
Catalog Number:
E21-R76-2Amount:
50ugSpecificity/Sensitivity:
2019-NcovPurity:
≥ 95% as determined by reducing SDS-PAGE. Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.Description:
Recombinant 2019-nCoV 3C-like Proteinase is produced by our E.coli expression system and the target gene encoding Ser1-Gln306 is expressed with a 6His tag at the N-terminus.Formulation:
Supplied as a 0.2 μm filtered solution of PBS, 1mM EDTA, 10% Glycerol, pH 7.4.Other Names:
M Proteinase; 3CL ProteinaseStorage/Stability:
Store at < -20°C, stable for 6 months after receipt.Background:
The viral main proteinase (M pro , also called 3CL pro ), which controls the activities of the coronavirus replication complex. It functions as a cysteine protease engaging in the proteolytic cleavage of the viral precursor polyprotein to a series of functional proteins required for coronavirus replication and is considered as an appealing target for designing anti-SARS agents.Molecular Weight:
35 kDaIsoelectric point:6.2Reaction:Dabcyl-KTSAVLQSGFRKME-Edans3CLP Dabcyl-KTSAVLQ + SGFRKME-EdansDefinition of activity:One unit is 1pmol sgfrkme-edans was catalyzed per minute at pH 7.0 and temperature 37°CActivity:
≥ 13000 u/mg
- Bioactivity test of Recombinant 2019-nCoV 3C-like Proteinase (N-6His) (CAT#E21-R76-2) measured by its ability to cleave a fluorogenic peptide substrate, Dabcyl-KTSAVLQSGFRKME-Edans (Synthetic) (CAT# EG214).The specific activity is >13000pmol/min/mg.
- Measurements of kinetic parameters of Recombinant 2019-nCoV 3C-like Proteinase (CAT#E21-R76-2) (For reference only).
Form:
Name:Recombinant 2019-nCoV 3C-like Proteinase (N-6His), Accession #: YP_009725301.1
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