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2019-nCoV & Monkeypox

Recombinant 2019-nCoV 3C-like Proteinase (N-6His)

E21-R76-2DatasheetCOAMSDS
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Specification
Quantity

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Specificty Sensitivity

Specificity/Sensitivity2019-Ncov

Product Information

Purity≥ 95% as determined by reducing SDS-PAGE. Endotoxin:  Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
DescriptionRecombinant 2019-nCoV 3C-like Proteinase is produced by our E.coli expression system and the target gene encoding Ser1-Gln306 is expressed with a 6His tag at the N-terminus.
FormulationSupplied as a 0.2 μm filtered solution of PBS, 1mM EDTA, 10% Glycerol, pH 7.4.
Molecular Weight35 kDaIsoelectric point:6.2Reaction:Dabcyl-KTSAVLQSGFRKME-Edans3CLP Dabcyl-KTSAVLQ + SGFRKME-EdansDefinition of activity:One unit is 1pmol sgfrkme-edans was catalyzed per minute at pH 7.0 and temperature 37°C
Activity≥ 13000 u/mg
FormName:Recombinant 2019-nCoV 3C-like Proteinase (N-6His), Accession #: YP_009725301.1
Other NamesM Proteinase; 3CL Proteinase
Storage/StabilityStore at < -20°C, stable for 6 months after receipt.
BackgroundThe viral main proteinase (M pro , also called 3CL pro ), which controls the activities of the coronavirus replication complex. It functions as a cysteine protease engaging in the proteolytic cleavage of the viral precursor polyprotein to a series of functional proteins required for coronavirus replication and is considered as an appealing target for designing anti-SARS agents.
Bioactivity test of Recombinant 2019-nCoV 3C-like Proteinase (N-6His) (CAT#E21-R76-2) measured ability to cleave a fluorogenic peptide substrate, Dabcyl-KTSAVLQSGFRKME-Edans (Synthetic) EG214).The specific activity is >13000pmol/min/mg.
Bioactivity test of Recombinant 2019-nCoV 3C-like Proteinase (N-6His) (CAT#E21-R76-2) measured ability to cleave a fluorogenic peptide substrate, Dabcyl-KTSAVLQSGFRKME-Edans (Synthetic) EG214).The specific activity is >13000pmol/min/mg.
Measurements of kinetic parameters of Recombinant 2019-nCoV 3C-like Proteinase (CAT#E21-R76-2) (For reference only).
Measurements of kinetic parameters of Recombinant 2019-nCoV 3C-like Proteinase (CAT#E21-R76-2) (For reference only).
📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E21-R76-2-Conjugate. Price: $485 / Size: 100μl.

E21-R76-2-FITC$485 / 100μlE21-R76-2-Biotin$485 / 100μlE21-R76-2-HRP$485 / 100μlE21-R76-2-AP$485 / 100μlE21-R76-2-AF350$485 / 100μlE21-R76-2-AF405M$485 / 100μlE21-R76-2-AF488$485 / 100μlE21-R76-2-AF514$485 / 100μlE21-R76-2-AF532$485 / 100μlE21-R76-2-AF555$485 / 100μlE21-R76-2-AF568$485 / 100μlE21-R76-2-AF405S$485 / 100μlE21-R76-2-AF405L$485 / 100μlE21-R76-2-AF546$485 / 100μlE21-R76-2-AF594$485 / 100μlE21-R76-2-AF610$485 / 100μlE21-R76-2-AF635$485 / 100μlE21-R76-2-AF647$485 / 100μlE21-R76-2-AF680$485 / 100μlE21-R76-2-AF700$485 / 100μlE21-R76-2-AF750$485 / 100μlE21-R76-2-AF790$485 / 100μlE21-R76-2-APC$485 / 100μlE21-R76-2-PE$485 / 100μlE21-R76-2-Cy3$485 / 100μlE21-R76-2-Cy5$485 / 100μlE21-R76-2-Cy5.5$485 / 100μlE21-R76-2-Cy7$485 / 100μlE21-R76-2-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.

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