Fibroblast Growth Factor 4/FGF-4
Datasheet
Catalog Number:
E21-R08Amount:
10ugPrice:
225$Altername:
Fibroblast growth factor 4; FGF-4; Heparin secretory-transforming protein 1; HST; HST-1; HSTF-1; Heparin-binding growth factor 4; HBGF-4; Transforming protein KS3; FGF4; HST; HSTF1; KS3Storage/Stability:
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Background:
Fibroblast growth factor 4(FGF-4) is a heparin binding member of the FGF family. The human FGF4 cDNA encodes 206 amino acids (aa) with a 33 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C-terminus. Mature human FGF4 shares 91%, 82%, 94% and 91% aa identity with mouse, rat, canine and bovine FGF4, respectively. Human FGF-4 has been shown to exhibit cross species activity. Expression of FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development. FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell selfrenewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro. FGF-4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential. It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease.Species:
HumanReconstitution:
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Endotoxin:
Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.Purity:
Greater than 95% as determined by reducing SDS-PAGE.Description:
Recombinant Human Fibroblast Growth Factor 4 is produced by our E.coli expression system and the target gene encoding Ser54-Leu206 is expressed.Product State:
LyophilizedShip Description:
The product is shipped at ambient temperature.Formulation:
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.Expression system:
E.coli
Greater than 95% as determined Measured in a by reducing SDS-PAGE. (QC cancer cells verified)
cell proliferation assay using MCF7 Human breast The ED50 for this effect is 2-20 ng/ml. (QC verified)
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Catalog Number
- Amount:10ug
- Price:225$
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