11β-HSD1 Polyclonal Antibody

Name: 11β-HSD1 Polyclonal Antibody
Brand: EnoGene Biotech Co. Ltd
SKU: E20-75560
Price: 225 USD
Availability: InStock

E20-75560Cite ID: EN0055693Datasheet COA MSDS

SourcePolyclonal, Rabbit

$225/ 100ul

Quantity

Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

OverviewImages & Data (2)Documents

Product Information

Molecular Weight32401Da

Protein NameCorticosteroid 11-beta-dehydrogenase isozyme 1

Human Gene Id3290

Human Swiss Prot NoP28845

Mouse Gene Id15483

Mouse Swiss Prot NoP50172

Rat Gene Id25116

Rat Swiss Prot NoP16232

Specificity11β-HSD1 Polyclonal Antibody detects endogenous levels of 11β-HSD1 protein.

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration1 mg/ml

Storage Stability-20°C/1 year

Other NameHSD11B1; HSD11; HSD11L; Corticosteroid 11-beta-dehydrogenase isozyme 1; 11-beta-hydroxysteroid dehydrogenase 1; 11-DH; 11-beta-HSD1

Observed Band(KD)35

Functioncatalytic activity:An 11-beta-hydroxysteroid + NADP(+) = an 11-oxosteroid + NADPH.,disease:Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).,function:Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol.,PTM:Glycosylated.,similarity:Belongs to the short-chain dehydrogenases/reductases (SDR) family.,subunit:Homodimer.,tissue specificity:Widely expressed. Highest expression in liver.,

Subcellular Locationendoplasmic reticulum membrane,membrane,integral component of membrane,

ExpressionBrain,Liver,Testis,

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ImmunogenSynthesized peptide derived from 11β-HSD1 . at AA range: internal

Gene NameHSD11B1

DilutionWestern Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.

Backgroundhydroxysteroid 11-beta dehydrogenase 1(HSD11B1) Homo sapiens The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Mutations in this gene and H6PD (hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)) are the cause of cortisone reductase deficiency. Alternate splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, May 2011],

📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E20-75560-Conjugate. Price: $485 / Size: 100μl.

E20-75560-FITC$485 / 100μlE20-75560-Biotin$485 / 100μlE20-75560-HRP$485 / 100μlE20-75560-AP$485 / 100μlE20-75560-AF350$485 / 100μlE20-75560-AF405M$485 / 100μlE20-75560-AF488$485 / 100μlE20-75560-AF514$485 / 100μlE20-75560-AF532$485 / 100μlE20-75560-AF555$485 / 100μlE20-75560-AF568$485 / 100μlE20-75560-AF405S$485 / 100μlE20-75560-AF405L$485 / 100μlE20-75560-AF546$485 / 100μlE20-75560-AF594$485 / 100μlE20-75560-AF610$485 / 100μlE20-75560-AF635$485 / 100μlE20-75560-AF647$485 / 100μlE20-75560-AF680$485 / 100μlE20-75560-AF700$485 / 100μlE20-75560-AF750$485 / 100μlE20-75560-AF790$485 / 100μlE20-75560-APC$485 / 100μlE20-75560-PE$485 / 100μlE20-75560-Cy3$485 / 100μlE20-75560-Cy5$485 / 100μlE20-75560-Cy5.5$485 / 100μlE20-75560-Cy7$485 / 100μlE20-75560-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.