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Cell Signal Transduction Research Antibodies
SPARC Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight33330Da
Protein NameSPARC
Human Gene Id6678
Human Swiss Prot NoP09486
Mouse Gene Id20692
Mouse Swiss Prot NoP07214
Rat Gene Id24791
Rat Swiss Prot NoP16975
SpecificitySPARC Polyclonal Antibody detects endogenous levels of SPARC protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameSPARC; ON; SPARC; Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine
Observed Band(KD)34
Functiondevelopmental stage:Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.,function:Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.,online information:Osteonectin entry,similarity:Belongs to the SPARC family.,similarity:Contains 1 EF-hand domain.,similarity:Contains 1 follistatin-like domain.,similarity:Contains 1 Kazal-like domain.,subcellular location:In or around the basement membrane.,
Subcellular Locationextracellular region,proteinaceous extracellular matrix,basement membrane,extracellular space,cytoplasm,mitochondrion,plasma membrane,cell surface,nuclear matrix,platelet alpha granule,platelet alpha granule membran
ExpressionMuscle,Placenta,PNS,Prostate,Skin,
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from the Internal region of human SPARC. AA range:141-190
Gene NameSPARC
DilutionWestern Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.
Backgroundsecreted protein acidic and cysteine rich(SPARC) Homo sapiens This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015],
Conjugated Antibody
This product is also available conjugated. Format: E20-75521-Conjugate. Price: $485 / Size: 100μl.
E20-75521-FITC$485 / 100μlE20-75521-Biotin$485 / 100μlE20-75521-HRP$485 / 100μlE20-75521-AP$485 / 100μlE20-75521-AF350$485 / 100μlE20-75521-AF405M$485 / 100μlE20-75521-AF488$485 / 100μlE20-75521-AF514$485 / 100μlE20-75521-AF532$485 / 100μlE20-75521-AF555$485 / 100μlE20-75521-AF568$485 / 100μlE20-75521-AF405S$485 / 100μlE20-75521-AF405L$485 / 100μlE20-75521-AF546$485 / 100μlE20-75521-AF594$485 / 100μlE20-75521-AF610$485 / 100μlE20-75521-AF635$485 / 100μlE20-75521-AF647$485 / 100μlE20-75521-AF680$485 / 100μlE20-75521-AF700$485 / 100μlE20-75521-AF750$485 / 100μlE20-75521-AF790$485 / 100μlE20-75521-APC$485 / 100μlE20-75521-PE$485 / 100μlE20-75521-Cy3$485 / 100μlE20-75521-Cy5$485 / 100μlE20-75521-Cy5.5$485 / 100μlE20-75521-Cy7$485 / 100μlE20-75521-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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