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Cell Signal Transduction Research Antibodies
POLR3G Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight27299Da
Protein NameDNA-directed RNA polymerase III subunit RPC7
Human Gene Id10622
Human Swiss Prot NoO15318
Mouse Gene Id67486
Mouse Swiss Prot NoQ6NXY9
SpecificityPOLR3G Polyclonal Antibody detects endogenous levels of POLR3G protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NamePOLR3G; DNA-directed RNA polymerase III subunit RPC7; RNA polymerase III subunit C7; DNA-directed RNA polymerase III subunit G; RNA polymerase III 32 kDa subunit; RPC32
Observed Band(KD)26
Functionfunction:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the RPC3/POLR3C-RPC6/POLR3F-RPC7/POLR3G subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation.,similarity:Belongs to the eukaryotic RPC7 RNA polymerase subunit family.,subunit:Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). RPC3/POLR3C, RPC6/POLR3F and RPC7/POLR3G form a Pol III subcomplex.,
Subcellular LocationNucleus . Cytoplasm . Excluded from nucleoli (PubMed:21898682). In zygotes and the 2-cell stage embryos, mainly in the cytoplasm. Starts to localize to the nucleus in the 8-16 cell stage embryo and early blastocysts (By similarity). .
ExpressionBarely detectable in differentiated tissues. Expressed in embryonic stem cells and in other dividing cells, such as some tumor cell lines.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from the Internal region of human POLR3G. AA range:151-200
Gene NamePOLR3G
DilutionWestern Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Backgroundfunction:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the RPC3/POLR3C-RPC6/POLR3F-RPC7/POLR3G subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation.,similarity:Belongs to the eukaryotic RPC7 RNA polymerase subunit family.,subunit:Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). RPC3/POLR3C, RPC6/POLR3F and RPC7/POLR3G form a Pol III subcomplex.,
Conjugated Antibody
This product is also available conjugated. Format: E20-75489-Conjugate. Price: $485 / Size: 100μl.
E20-75489-FITC$485 / 100μlE20-75489-Biotin$485 / 100μlE20-75489-HRP$485 / 100μlE20-75489-AP$485 / 100μlE20-75489-AF350$485 / 100μlE20-75489-AF405M$485 / 100μlE20-75489-AF488$485 / 100μlE20-75489-AF514$485 / 100μlE20-75489-AF532$485 / 100μlE20-75489-AF555$485 / 100μlE20-75489-AF568$485 / 100μlE20-75489-AF405S$485 / 100μlE20-75489-AF405L$485 / 100μlE20-75489-AF546$485 / 100μlE20-75489-AF594$485 / 100μlE20-75489-AF610$485 / 100μlE20-75489-AF635$485 / 100μlE20-75489-AF647$485 / 100μlE20-75489-AF680$485 / 100μlE20-75489-AF700$485 / 100μlE20-75489-AF750$485 / 100μlE20-75489-AF790$485 / 100μlE20-75489-APC$485 / 100μlE20-75489-PE$485 / 100μlE20-75489-Cy3$485 / 100μlE20-75489-Cy5$485 / 100μlE20-75489-Cy5.5$485 / 100μlE20-75489-Cy7$485 / 100μlE20-75489-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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