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Cell Signal Transduction Research Antibodies
NFκB-p65 Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight60219Da
Protein NameTranscription factor p65
Human Gene Id5970
Human Swiss Prot NoQ04206
Mouse Gene Id19697
Mouse Swiss Prot NoQ04207
SpecificityNFκB-p65 Polyclonal Antibody detects endogenous levels of NFκB-p65 protein only when acetylation at K314/K315.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameRELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Functionfunction:NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by in
Subcellular LocationNucleus . Cytoplasm . Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B) (PubMed:1493333). Colocalized with DDX1 in the nucleus upon TNF-alpha induction (PubMed:19058135). Colocalizes with GFI1 in the nucleus after LPS stimulation (PubMed:20547752). Translocation to the nucleus is impaired in L.monocytogenes infection (PubMed:20855622). .
ExpressionBone,Colon,Pancreas,Placenta,
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenSynthesized peptide derived from human NFκB-p65 around the non-acetylation site of K314/K315.
Gene NameRELA
DilutionWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
BackgroundRELA proto-oncogene, NF-kB subunit(RELA) Homo sapiens NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],
Conjugated Antibody
This product is also available conjugated. Format: E20-75428-Conjugate. Price: $485 / Size: 100μl.
E20-75428-FITC$485 / 100μlE20-75428-Biotin$485 / 100μlE20-75428-HRP$485 / 100μlE20-75428-AP$485 / 100μlE20-75428-AF350$485 / 100μlE20-75428-AF405M$485 / 100μlE20-75428-AF488$485 / 100μlE20-75428-AF514$485 / 100μlE20-75428-AF532$485 / 100μlE20-75428-AF555$485 / 100μlE20-75428-AF568$485 / 100μlE20-75428-AF405S$485 / 100μlE20-75428-AF405L$485 / 100μlE20-75428-AF546$485 / 100μlE20-75428-AF594$485 / 100μlE20-75428-AF610$485 / 100μlE20-75428-AF635$485 / 100μlE20-75428-AF647$485 / 100μlE20-75428-AF680$485 / 100μlE20-75428-AF700$485 / 100μlE20-75428-AF750$485 / 100μlE20-75428-AF790$485 / 100μlE20-75428-APC$485 / 100μlE20-75428-PE$485 / 100μlE20-75428-Cy3$485 / 100μlE20-75428-Cy5$485 / 100μlE20-75428-Cy5.5$485 / 100μlE20-75428-Cy7$485 / 100μlE20-75428-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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