EKLF Polyclonal Antibody

Name: EKLF Polyclonal Antibody
Brand: EnoGene Biotech Co. Ltd
SKU: E20-75425
Price: 225 USD
Availability: InStock

E20-75425Cite ID: EN0055558Datasheet COA MSDS

SourcePolyclonal, Rabbit

$225/ 100ul

Quantity

Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

OverviewImages & Data (2)Documents

Product Information

Molecular Weight38221Da

Protein NameKrueppel-like factor 1

Human Gene Id10661

Human Swiss Prot NoQ13351

Mouse Swiss Prot NoP46099

SpecificityEKLF Polyclonal Antibody detects endogenous levels of EKLF protein only when acetylation at K274.

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration1 mg/ml

Storage Stability-20°C/1 year

Other NameKLF1; EKLF; Krueppel-like factor 1; Erythroid krueppel-like transcription factor; EKLF

Observed Band(KD)38

Functionfunction:Transcription regulator of erythrocyte development. Binds to the CACCC box in the beta-globin gene promoter and activates transcription. When sumoylation, acts as a Probably serves as a general switch factor for erythroid development. When sumoylated, acts as a transcriptional repressor, by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.,PTM:Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity.,PTM:Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity.,PTM:Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex.,simila

Subcellular LocationNucleus . Colocalizes with SUMO1 in nuclear speckles. .

ExpressionExpression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines.

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ImmunogenThe antiserum was produced against synthesized peptide derived from human KLF1 around the non-acetylation site of Lys274. AA range:231-280

Gene NameKLF1

DilutionWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

BackgroundKruppel like factor 1(KLF1) Homo sapiens This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. [provided by RefSeq, Oct 2009],

📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E20-75425-Conjugate. Price: $485 / Size: 100μl.

E20-75425-FITC$485 / 100μlE20-75425-Biotin$485 / 100μlE20-75425-HRP$485 / 100μlE20-75425-AP$485 / 100μlE20-75425-AF350$485 / 100μlE20-75425-AF405M$485 / 100μlE20-75425-AF488$485 / 100μlE20-75425-AF514$485 / 100μlE20-75425-AF532$485 / 100μlE20-75425-AF555$485 / 100μlE20-75425-AF568$485 / 100μlE20-75425-AF405S$485 / 100μlE20-75425-AF405L$485 / 100μlE20-75425-AF546$485 / 100μlE20-75425-AF594$485 / 100μlE20-75425-AF610$485 / 100μlE20-75425-AF635$485 / 100μlE20-75425-AF647$485 / 100μlE20-75425-AF680$485 / 100μlE20-75425-AF700$485 / 100μlE20-75425-AF750$485 / 100μlE20-75425-AF790$485 / 100μlE20-75425-APC$485 / 100μlE20-75425-PE$485 / 100μlE20-75425-Cy3$485 / 100μlE20-75425-Cy5$485 / 100μlE20-75425-Cy5.5$485 / 100μlE20-75425-Cy7$485 / 100μlE20-75425-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.