Histone H4 Polyclonal Antibody

Name: Histone H4 Polyclonal Antibody
Brand: EnoGene Biotech Co. Ltd
SKU: E20-75422

E20-75422Cite ID: EN0055555Datasheet COA MSDS

SourcePolyclonal, Rabbit

Price on request

Quantity

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

OverviewImages & Data (2)Documents

Product Information

Molecular Weight11367Da

Protein NameHistone H4

Human Gene Id121504/554313/8294/8359/8360/8361/8362/8363/8364/8365/8366/8367/8368/8370

Human Swiss Prot NoP62805

Mouse Gene Id100041230

Mouse Swiss Prot NoP62806

Rat Gene Id100360950

Rat Swiss Prot NoP62804

SpecificityHistone H4 Polyclonal Antibody detects endogenous levels of Histone H4 protein only when acetylation at K92.

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration1 mg/ml

Storage Stability-20°C/1 year

Other NameHIST1H4A; H4/A; H4FA; HIST1H4B; H4/I; H4FI; HIST1H4C; H4/G; H4FG; HIST1H4D; H4/B; H4FB; HIST1H4E; H4/J; H4FJ; HIST1H4F; H4/C; H4FC; HIST1H4H; H4/H; H4FH; HIST1H4I; H4/M; H4FM; HIST1H4J; H4/E; H4FEHIST1H4K; H4/D; H4FD; HIST1H4L; H4/K; H4FK; HIST2H4A; H4/N; H4F2; H4FN; HIST2H4; HIST2H4B; H4/O; H4FO; HIST4H4; Histone H4

Observed Band(KD)15

Functionfunction:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,PTM:Acetylation at Lys-6, Lys-9, Lys-13 and Lys-17 occurs in coding regions of the genome but not in heterochromatin.,PTM:Citrullination at Arg-4 by PADI4 impairs methylation.,PTM:Monomethylated, dimethylated or trimethylated at Lys-21. Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.,PTM:Monomethylation at Arg-4 by PRMT1 favors acetylation at Lys-9 and Lys-13. Demethylation is p

Subcellular Locationnuclear chromosome,nuclear chromosome, telomeric region,nucleosome,extracellular region,nucleus,nucleoplasm,membrane,extracellular matrix,protein complex,extracellular exosome,

ExpressionB-cell lymphoma,Bone marrow,Brain,Clones donated by HIP,Corpus call

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ImmunogenSynthesized peptide derived from human Histone H4 around the non-acetylation site of K92.

Gene NameHIST1H4A

DilutionWestern Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.

Backgroundhistone cluster 1 H4 family member i(HIST1H4I) Homo sapiens Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015],

📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E20-75422-Conjugate. Price: $485 / Size: 100μl.

E20-75422-FITC$485 / 100μlE20-75422-Biotin$485 / 100μlE20-75422-HRP$485 / 100μlE20-75422-AP$485 / 100μlE20-75422-AF350$485 / 100μlE20-75422-AF405M$485 / 100μlE20-75422-AF488$485 / 100μlE20-75422-AF514$485 / 100μlE20-75422-AF532$485 / 100μlE20-75422-AF555$485 / 100μlE20-75422-AF568$485 / 100μlE20-75422-AF405S$485 / 100μlE20-75422-AF405L$485 / 100μlE20-75422-AF546$485 / 100μlE20-75422-AF594$485 / 100μlE20-75422-AF610$485 / 100μlE20-75422-AF635$485 / 100μlE20-75422-AF647$485 / 100μlE20-75422-AF680$485 / 100μlE20-75422-AF700$485 / 100μlE20-75422-AF750$485 / 100μlE20-75422-AF790$485 / 100μlE20-75422-APC$485 / 100μlE20-75422-PE$485 / 100μlE20-75422-Cy3$485 / 100μlE20-75422-Cy5$485 / 100μlE20-75422-Cy5.5$485 / 100μlE20-75422-Cy7$485 / 100μlE20-75422-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.